Literature DB >> 12173769

The propagation of a porcine epidemic diarrhea virus in swine cell lines.

K Kadoi1, H Sugioka, T Satoh, B K Kadoi.   

Abstract

A strain of porcine epidemic diarrhea virus (PEDV), P-5V, utilized as a live virus vaccine in Japan was infected to a swine cell lines, KSEK6 and IB-RS-2 cells. Clear CPE, characterized by cellular destruction, started to appear in the infected cells on 2-3 days post infection (DPI) and affected cells was completely degenerated on 4 DPI. The virus was serially passaged in the cells even without addition of trypsin. Small but clear plaques were formed under an agar overlay medium on the cells. The infective titer in the order of 10(7.00-7.50) TCID50 per ml was obtained at usual incubation temperature.

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Year:  2002        PMID： 12173769

Source DB:  PubMed          Journal:  New Microbiol        ISSN： 1121-7138            Impact factor:   2.479


  8 in total

1.  Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China.

Authors:  Zhi-Li Li; Ling Zhu; Jing-Yun Ma; Qing-Feng Zhou; Yan-Hua Song; Bao-Li Sun; Rui-Ai Chen; Qing-Mei Xie; Ying-Zuo Bee
Journal:  Virus Genes       Date:  2012-04-12       Impact factor: 2.332

2.  Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain.

Authors:  D S Song; J S Oh; B K Kang; J S Yang; H J Moon; H S Yoo; Y S Jang; B K Park
Journal:  Res Vet Sci       Date:  2006-05-30       Impact factor: 2.534

3.  Probiotic supplementation improves reproductive performance of unvaccinated farmed sows infected with porcine epidemic diarrhea virus.

Authors:  Takamitsu Tsukahara; Takio Inatomi; Konosuke Otomaru; Masaaki Amatatsu; Gustavo A Romero-Pérez; Ryo Inoue
Journal:  Anim Sci J       Date:  2018-05-27       Impact factor: 1.749

Review 4.  Emerging Highly Virulent Porcine Epidemic Diarrhea Virus: Molecular Mechanisms of Attenuation and Rational Design of Live Attenuated Vaccines.

Authors:  Yixuan Hou; Qiuhong Wang
Journal:  Int J Mol Sci       Date:  2019-11-04       Impact factor: 5.923

Review 5.  Modulation of Innate Antiviral Immune Response by Porcine Enteric Coronavirus.

Authors:  Kunli Zhang; Sen Lin; Jianhao Li; Shoulong Deng; Jianfeng Zhang; Sutian Wang
Journal:  Front Microbiol       Date:  2022-02-14       Impact factor: 5.640

6.  Effective inhibition of porcine epidemic diarrhea virus by RNA interference in vitro.

Authors:  Haiyan Shen; Chunhong Zhang; Pengju Guo; Zhicheng Liu; Jianfeng Zhang
Journal:  Virus Genes       Date:  2015-09-02       Impact factor: 2.332

7.  Application of a focus formation assay for detection and titration of porcine epidemic diarrhea virus.

Authors:  Deu John M Cruz; Hyun-Jin Shin
Journal:  J Virol Methods       Date:  2007-06-18       Impact factor: 2.014

Review 8.  Deciphering the biology of porcine epidemic diarrhea virus in the era of reverse genetics.

Authors:  Samaporn Teeravechyan; Phanramphoei Namprachan Frantz; Phonphimon Wongthida; Thanathom Chailangkarn; Peera Jaru-Ampornpan; Surapong Koonpaew; Anan Jongkaewwattana
Journal:  Virus Res       Date:  2016-05-20       Impact factor: 3.303
  8 in total

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