Ken-ichi Watanabe1, Ken Jinnouchi, Toshiaki Yagi. 1. Department of Oto-Rhino-Laryngology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan. bxp0264@nifty.ne.jp
Abstract
OBJECTIVE: In this study, the effect of endotoxin on the vestibule of the ear of guinea pigs was immunohistochemically examined. METHODS: Bacterial lipopolysaccharide was injected into the middle ear transtympanically. After 48 h, the animals were sacrificed by intracardiac perfusion of fixative; then, the temporal bones were removed and processed for immunohistochemical staining with the anti-myeloperoxidase antibody. RESULTS: Myeloperoxidase could be detected after 48 h in the sensory epithelium and the dark cell area. CONCLUSION: It is reported that radical oxygen species, which are cytotoxic, are detected under inflammatory conditions. Our results suggest that myeloperoxidase and reactive oxygen species are involved in vestibular dysfunction under inflammation.
OBJECTIVE: In this study, the effect of endotoxin on the vestibule of the ear of guinea pigs was immunohistochemically examined. METHODS: Bacterial lipopolysaccharide was injected into the middle ear transtympanically. After 48 h, the animals were sacrificed by intracardiac perfusion of fixative; then, the temporal bones were removed and processed for immunohistochemical staining with the anti-myeloperoxidase antibody. RESULTS:Myeloperoxidase could be detected after 48 h in the sensory epithelium and the dark cell area. CONCLUSION: It is reported that radical oxygen species, which are cytotoxic, are detected under inflammatory conditions. Our results suggest that myeloperoxidase and reactive oxygen species are involved in vestibular dysfunction under inflammation.
Authors: Ozlem Celebi; Mustafa Paksoy; Sedat Aydin; Arif Sanlı; Omer Taşdemir; Aylin Ege Gül Journal: Indian J Otolaryngol Head Neck Surg Date: 2010-06-04