| Literature DB >> 12162562 |
Seiji Oyama1, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima.
Abstract
A gene (neg1) encoding an endo-1,6-beta-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-beta-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-beta-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.Entities:
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Year: 2002 PMID： 12162562 DOI： 10.1271/bbb.66.1378
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043