BACKGROUND: Cancer cells require more cholesterol than normal cells. This requirement may be satisfied by higher hydroxymethylglutaryl coenzyme A reductase activity or low-density lipoprotein receptor expression or both. We investigated hydroxymethylglutaryl coenzyme A reductase activity and low-density lipoprotein receptor expression in samples of gastric carcinoma and normal mucosa obtained from 30 patients. The samples of gastric carcinoma were divided into two categories according to Lauren's classification: diffuse-type and intestinal-type. METHODS: Hydroxymethylglutaryl coenzyme A reductase activity was measured by radiometric assay and low-density lipoprotein receptor expression was measured by enzyme-linked immunosorbent assay. RESULTS: Hydroxy-methyl-glutaryl-coenzyme A reductase activity was significantly higher in neoplastic tissue than in normal mucosa, both in diffuse-type and intestinal-type gastric cancer. Low-density lipoprotein receptor levels were significantly lower in neoplastic tissue than in normal mucosa of diffuse-type gastric cancer, whereas no significant difference was present between neoplastic tissue and normal mucosa in intestinal-type gastric cancer. Hydroxymethylglutaryl coenzyme A reductase activity did not differ between intestinal-type and diffuse-type neoplastic samples, while low-density lipoprotein receptor levels were significantly higher in intestinal-type than in diffuse-type gastric cancer tissue. CONCLUSIONS: These findings show different behavior of cholesterol metabolism in the two types of gastric carcinoma and, therefore, suggest that different therapeutic strategies should be adopted.
BACKGROUND: Cancer cells require more cholesterol than normal cells. This requirement may be satisfied by higher hydroxymethylglutaryl coenzyme A reductase activity or low-density lipoprotein receptor expression or both. We investigated hydroxymethylglutaryl coenzyme A reductase activity and low-density lipoprotein receptor expression in samples of gastric carcinoma and normal mucosa obtained from 30 patients. The samples of gastric carcinoma were divided into two categories according to Lauren's classification: diffuse-type and intestinal-type. METHODS: Hydroxymethylglutaryl coenzyme A reductase activity was measured by radiometric assay and low-density lipoprotein receptor expression was measured by enzyme-linked immunosorbent assay. RESULTS: Hydroxy-methyl-glutaryl-coenzyme A reductase activity was significantly higher in neoplastic tissue than in normal mucosa, both in diffuse-type and intestinal-type gastric cancer. Low-density lipoprotein receptor levels were significantly lower in neoplastic tissue than in normal mucosa of diffuse-type gastric cancer, whereas no significant difference was present between neoplastic tissue and normal mucosa in intestinal-type gastric cancer. Hydroxymethylglutaryl coenzyme A reductase activity did not differ between intestinal-type and diffuse-type neoplastic samples, while low-density lipoprotein receptor levels were significantly higher in intestinal-type than in diffuse-type gastric cancer tissue. CONCLUSIONS: These findings show different behavior of cholesterol metabolism in the two types of gastric carcinoma and, therefore, suggest that different therapeutic strategies should be adopted.
Authors: Gemma Llaverias; Christiane Danilo; Isabelle Mercier; Kristin Daumer; Franco Capozza; Terence M Williams; Federica Sotgia; Michael P Lisanti; Philippe G Frank Journal: Am J Pathol Date: 2010-12-23 Impact factor: 4.307
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Authors: Maria Notarnicola; Maria Gabriella Caruso; Angela Tafaro; Valeria Tutino; Giusy Bianco; Mario Minoia; Antonio Francavilla Journal: World J Gastroenterol Date: 2013-12-14 Impact factor: 5.742
Authors: Alfonso Dueñas-González; Patricia García-López; Luis Alonso Herrera; Jose Luis Medina-Franco; Aurora González-Fierro; Myrna Candelaria Journal: Mol Cancer Date: 2008-10-23 Impact factor: 27.401