| Literature DB >> 12150943 |
Alan T Remaley1, Samantha Bark, Avram D Walts, Lita Freeman, Sergey Shulenin, Tarmo Annilo, Eric Elgin, Hope E Rhodes, Charles Joyce, Michael Dean, Silvia Santamarina-Fojo, H Bryan Brewer.
Abstract
The excretion of sterols from the liver and intestine is regulated by the ABCG5 and ABCG8 transporters. To identify potential regulatory elements, 152 kb of the human ABCG5-ABCG8 gene cluster was sequenced and comparative genome analysis was performed. The two genes are oriented in a head-to-head configuration and are separated by a 374-bp intergenic region, which is highly conserved among several species. Using a reporter construct, the intergenic region was found to act as a bidirectional promoter. A conserved GATA site in the intergenic region was shown by site-directed mutagenesis to act as a repressor for the ABCG5 promoter. The intergenic region was also shown to be partially responsive to treatment by LXR agonists. In summary, several potential regulatory elements were found for the ABCG5 and ABCG8 genes, and the intergenic region was found to act as a bidirectional promoter.Entities:
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Year: 2002 PMID： 12150943 DOI： 10.1016/s0006-291x(02)00652-6
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575