Immunogenicity of inactivated influenza vaccine, purified by adsorption chromatography on porous glas.

A chromatographic process has been developed for a mass purification and concentration of influenza virus. The final virus suspension, purified some 400 times, is characterized by its antigenic properties and protein content. The virus was killed by UV-light and the resulting vaccine tried on 345 people. The immunity level is 8 times higher than the control. In mice which were infected after immunization with a homologous and heterologous influenza virus strains a high protection index was found (10(6) EID50 for homologous and 10(4) EID50 for heterologous strains).