Literature DB >> 12144509

Production of recombinant human calcitonin from silkworm (B. mori) larvae infected by baculovirus.

Guan-Zhen Yang1, Zhen-zhen Chen, Da-Fu Cui, Bo-liang Li, Xiang-fu Wu.   

Abstract

A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

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Year:  2002        PMID: 12144509     DOI: 10.2174/0929866023408689

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN: 0929-8665            Impact factor:   1.890


  1 in total

1.  Production of the catalytic core of human peptidylglycine α-hydroxylating monooxygenase (hPHMcc) in Escherichia coli.

Authors:  Sumit Handa; Tyler J Spradling; Daniel R Dempsey; David J Merkler
Journal:  Protein Expr Purif       Date:  2012-04-25       Impact factor: 1.650

  1 in total

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