PURPOSE: To develop a rat model to experimentally monitor the potential inflammatory effects of soft contact lens (CL) usage on the cornea during extended wear (EW). METHODS: Lewis rats were fitted with EW lotrafilcon A (CIBA Vision, Duluth, GA) hydrogel lenses (Dk/t 175 barrers/cm) in the left eye, the right eye serving as a control. After 12 days (n = 5 rats) and 30 days (n = 8 rats) of continuous extended wear, corneas were removed and total RNA was extracted from both CL-wearing and non-lens-wearing eyes. Multiprobe ribonuclease protection assays (RPA) were used to detect and compare cytokine and chemokine gene expression in corneas from both groups. RESULTS: Cytokine-chemokine mRNA expression levels were similar for interleukin (IL)-1alpha, IL-1 receptor antagonist (RA), IL- 18, transforming growth factor (TGF)-beta1, TGF-beta2, TGF-beta3, and macrophage migration inhibitory factor (MIF) levels in CL-wearing and non-lens-wearing corneas after 12 or 30 days of EW. CONCLUSION: This in vivo rat model for extended contact lens wear allows analysis and comparison of mRNA levels of cytokines and chemokines in the cornea with and without EW soft CL use. Remarkably, after 12 or 30 days of continuous CL wear, there was no significant up-regulation in lens wearing corneas for any of the cytokines-chemokines tested.
PURPOSE: To develop a rat model to experimentally monitor the potential inflammatory effects of soft contact lens (CL) usage on the cornea during extended wear (EW). METHODS: Lewis rats were fitted with EW lotrafilcon A (CIBA Vision, Duluth, GA) hydrogel lenses (Dk/t 175 barrers/cm) in the left eye, the right eye serving as a control. After 12 days (n = 5 rats) and 30 days (n = 8 rats) of continuous extended wear, corneas were removed and total RNA was extracted from both CL-wearing and non-lens-wearing eyes. Multiprobe ribonuclease protection assays (RPA) were used to detect and compare cytokine and chemokine gene expression in corneas from both groups. RESULTS: Cytokine-chemokine mRNA expression levels were similar for interleukin (IL)-1alpha, IL-1 receptor antagonist (RA), IL- 18, transforming growth factor (TGF)-beta1, TGF-beta2, TGF-beta3, and macrophage migration inhibitory factor (MIF) levels in CL-wearing and non-lens-wearing corneas after 12 or 30 days of EW. CONCLUSION: This in vivo rat model for extended contact lens wear allows analysis and comparison of mRNA levels of cytokines and chemokines in the cornea with and without EW soft CL use. Remarkably, after 12 or 30 days of continuous CL wear, there was no significant up-regulation in lens wearing corneas for any of the cytokines-chemokines tested.
Authors: Fiona Stapleton; Carl Marfurt; Blanka Golebiowski; Mark Rosenblatt; David Bereiter; Carolyn Begley; Darlene Dartt; Juana Gallar; Carlos Belmonte; Pedram Hamrah; Mark Willcox Journal: Invest Ophthalmol Vis Sci Date: 2013-10-18 Impact factor: 4.799
Authors: Matteo M E Metruccio; Stephanie J Wan; Hart Horneman; Abby R Kroken; Aaron B Sullivan; Tan N Truong; James J Mun; Connie K P Tam; Robin Frith; Laurence Welsh; Melanie D George; Carol A Morris; David J Evans; Suzanne M J Fleiszig Journal: Ocul Surf Date: 2018-11-12 Impact factor: 5.033
Authors: Yunfan Zhang; Manal M Gabriel; Mary F Mowrey-McKee; Ronald P Barrett; Sharon McClellan; Linda D Hazlett Journal: Eye Contact Lens Date: 2008-11 Impact factor: 2.018
Authors: Suzanne M J Fleiszig; Abby R Kroken; Vincent Nieto; Melinda R Grosser; Stephanie J Wan; Matteo M E Metruccio; David J Evans Journal: Prog Retin Eye Res Date: 2019-11-20 Impact factor: 21.198