Two-hybrid systems and their usage in infection biology.

Two-hybrid systems are powerful tools to study protein-protein interactions in biological systems. The role of protein-protein interactions involved in pathogenesis of bacterial and viral infections were defined by using yeast or bacterial two-hybrid screens. Examples are given to highlight the specificity of interactions in signaling pathways, in regulation, secretion and structure-function relationships of virulence factors and their cellular targets. Two-hybrid systems were also used to establish large-scale protein interaction maps of viral and bacterial pathogens, that might be useful to identify targets for new drugs or vaccines.