OBJECTIVE: To investigate whether hCG may directly influence endometrial differentiation and function. DESIGN: Controlled clinical study. SETTING: Tertiary university center. PATIENT(S): Fifty-six women with infertility. INTERVENTION(S): An intrauterine microdialysis device (IUMD) was developed that consisted of two balloon catheters connected by microdialysis tubing (molecular weight cutoff: 2,000 kDa). The IUMD was inserted into the uterine cavity and perfused with saline for 3 hours. In 45 women, urinary hCG was then added for 5 hours. Eleven women underwent an identical procedure but without the application of hCG. MAIN OUTCOME MEASURE(S): The response of the endometrium was assessed by measuring IGFBP-1 in the perfusate. RESULT(S): Intrauterine secretion of IGFBP-1 was strictly confined to the late secretory phase (>or=10 days after the beginning of the LH peak). This time point marks the closing of the implantation window. The application of hCG did not affect intrauterine IGFBP-1 levels before day 10 but induced a significant decrease of intrauterine IGFBP-1 levels thereafter. There was no significant change of intrauterine IGFBP-1 levels in the controls. CONCLUSION(S): Intrauterine microdialysis allows a dynamic assessment of endometrial paracrine function in vivo. Human chorionic gonadotropin may be involved in the mechanisms regulating endometrial receptivity.
OBJECTIVE: To investigate whether hCG may directly influence endometrial differentiation and function. DESIGN: Controlled clinical study. SETTING: Tertiary university center. PATIENT(S): Fifty-six women with infertility. INTERVENTION(S): An intrauterine microdialysis device (IUMD) was developed that consisted of two balloon catheters connected by microdialysis tubing (molecular weight cutoff: 2,000 kDa). The IUMD was inserted into the uterine cavity and perfused with saline for 3 hours. In 45 women, urinary hCG was then added for 5 hours. Eleven women underwent an identical procedure but without the application of hCG. MAIN OUTCOME MEASURE(S): The response of the endometrium was assessed by measuring IGFBP-1 in the perfusate. RESULT(S): Intrauterine secretion of IGFBP-1 was strictly confined to the late secretory phase (>or=10 days after the beginning of the LH peak). This time point marks the closing of the implantation window. The application of hCG did not affect intrauterine IGFBP-1 levels before day 10 but induced a significant decrease of intrauterine IGFBP-1 levels thereafter. There was no significant change of intrauterine IGFBP-1 levels in the controls. CONCLUSION(S): Intrauterine microdialysis allows a dynamic assessment of endometrial paracrine function in vivo. Human chorionic gonadotropin may be involved in the mechanisms regulating endometrial receptivity.
Authors: Lidia Boldeanu; Anda Lorena Dijmărescu; Mirela Radu; Cristian Adrian Siloşi; Mircea Vasile Popescu-Drigă; Ioan Sabin Poenariu; Isabela Siloşi; Mihail Virgil Boldeanu; Marius Bogdan Novac; Liliana Victoria Novac Journal: Rom J Morphol Embryol Date: 2020 Jul-Sep Impact factor: 1.033