| Literature DB >> 12137509 |
Marie-Laure Lesaicherre1, Rina Y P Lue, Grace Y J Chen, Qing Zhu, Shao Q Yao.
Abstract
We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays.Entities:
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Year: 2002 PMID： 12137509 DOI： 10.1021/ja0265963
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419