| Literature DB >> 12135426 |
L-H Tseng1, P-J Chen, M-T Lin, K Singleton, E G Martin, A-H Yen, S-M Chuang, P J Martin, J A Hansen.
Abstract
Single nucleotide polymorphisms (SNP) in the human IL-6, IL-10, TNFalpha and TNFbeta genes have been associated with gene function and susceptibility to disease. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a new restriction site recognized by endonucleases AlwNI, BcgI, BglI, BsaBI, BslI, BstXI, EcoNI or XcmI for genotyping SNPs in the IL-6 gene (position - 174), IL-10 gene (positions -592 and -1082), TNFalpha gene (positions -238, - 308 and -863) and TNFbeta gene (position + 249) by mismatched polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). Our results show that appropriately designed BslI-based mismatched PCR/RFLP assays can be successfully used to determine the genotypes for approximately 40% of SNPs. The mismatched PCR strategy can be coupled with multiplex-amplification to enable simple and rapid determination of several SNP genotypes in a single reaction.Entities:
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Year: 2002 PMID： 12135426 DOI： 10.1034/j.1399-0039.2002.590405.x
Source DB: PubMed Journal: Tissue Antigens ISSN： 0001-2815