INTRODUCTION: Glia conditioned medium (GCM) is neurotrophic for dopaminergic (DA) neurons and protects against MPP+ toxicity in vitro. We present the data from the first study in vivo of the effects of GCM. MATERIAL AND METHODS: The restorative effects were examined in rats with lesions produced by 6-hydroxy-dopamine (6-OH-DA) in the medial longitudinal fasciculus. At four weeks, animals with an apomorphine induced rotation rate above three per minute were randomised for infusion with GCM, defined medium (DM) or saline through a striatal cannula for two weeks. To investigate the protective effects of GCM, the animals received a striatal injection of GCM or vehicle at the same time as the lesion was induced by 6-OH-DA and were sacrificed three weeks later. RESULTS: In the experiments on the restorative effect, the GCM infusion produced a significant increase in dopamine (DA) levels on the side with the lesion, in the limbic system (455.8 108.4 ng/g of tissue) and in the striatum (242.1 69.2 ng/g of tissue), as compared with the controls (110.8 36,4 and 108.4 22 ng/g). In the experiments on the protective effect, GCM induced higher levels of DA, 3,4-dihydroxyphenylacetic acid and 3 methoxytyramine (3-MT). In both models, the apomorphine induced rotation in animals with lesions caused by 6-OH-DA and treated with GCM was lower than that of the animals infused with DM or saline. CONCLUSIONS: These experiments show that GCM has protective and restorative effects in an experimental model of Parkinson's disease.
INTRODUCTION:Glia conditioned medium (GCM) is neurotrophic for dopaminergic (DA) neurons and protects against MPP+toxicity in vitro. We present the data from the first study in vivo of the effects of GCM. MATERIAL AND METHODS: The restorative effects were examined in rats with lesions produced by 6-hydroxy-dopamine (6-OH-DA) in the medial longitudinal fasciculus. At four weeks, animals with an apomorphine induced rotation rate above three per minute were randomised for infusion with GCM, defined medium (DM) or saline through a striatal cannula for two weeks. To investigate the protective effects of GCM, the animals received a striatal injection of GCM or vehicle at the same time as the lesion was induced by 6-OH-DA and were sacrificed three weeks later. RESULTS: In the experiments on the restorative effect, the GCM infusion produced a significant increase in dopamine (DA) levels on the side with the lesion, in the limbic system (455.8 108.4 ng/g of tissue) and in the striatum (242.1 69.2 ng/g of tissue), as compared with the controls (110.8 36,4 and 108.4 22 ng/g). In the experiments on the protective effect, GCM induced higher levels of DA, 3,4-dihydroxyphenylacetic acid and 3 methoxytyramine (3-MT). In both models, the apomorphine induced rotation in animals with lesions caused by 6-OH-DA and treated with GCM was lower than that of the animals infused with DM or saline. CONCLUSIONS: These experiments show that GCM has protective and restorative effects in an experimental model of Parkinson's disease.
Authors: Juan Perucho; Maria José Casarejos; Ana Gómez; Carolina Ruíz; Maria Ángeles Fernández-Estevez; Maria Paz Muñoz; Justo García de Yébenes; Maria Ángeles Mena Journal: PLoS One Date: 2013-09-12 Impact factor: 3.240