Wenyu Fu1, Yanmeng Lu, Yingjie Piao. 1. Central Laboratory, The First Military Medical University, Guangzhou 510515, China.
Abstract
OBJECTIVE: To cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality. METHODS: Bone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks. RESULTS: Human fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue. CONCLUSION: Human fetal marrow MSCs were multipotent stem cells.
OBJECTIVE: To cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality. METHODS: Bone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks. RESULTS:Human fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue. CONCLUSION:Human fetal marrow MSCs were multipotent stem cells.
Authors: Chunfeng Zhao; Hsiao-Feng Chieh; Karim Bakri; Jun Ikeda; Yu-Long Sun; Steven L Moran; Kai-Nan An; Peter C Amadio Journal: Med Eng Phys Date: 2009-09-06 Impact factor: 2.242