OBJECTIVE: To evaluate the effect of dipyridamole (DPM) and adenosine (ADO) on interstitial pulmonary fibrosis in mice. METHODS: 100 mice injected with Bleomycin (BLM, 8.5 mg/kg) by intratracheal were divided into a control group and groups treated by DPM, ADO, DPM and theophyline (TH). Pulmonary pathology from day 4 to day 30 observations was studied by light and electron microscope. RESULTS: Alveolar epithelial cell necrosis, lung tissue lesions by hypoxia and interstitial fibrosis were found in the BLM group after day 10. The necrotic epithelial cell, fibroblast proliferation, and the hypoxia induced tissue damage were less prominent, and the number and phagocytic function of macrophages were increased in the DPM and ADO treated groups. In the TH treated group, necrotic epithelial cells increased, the lung tissue was ischemic, and the differentiation of monocytes in pulmonary interstitial was slower. CONCLUSION: DPM and ADO can inhibit fibroblast proliferation by improving microcirculation and promoting macrophage development and alveolar space reconstruction.
OBJECTIVE: To evaluate the effect of dipyridamole (DPM) and adenosine (ADO) on interstitial pulmonary fibrosis in mice. METHODS: 100 mice injected with Bleomycin (BLM, 8.5 mg/kg) by intratracheal were divided into a control group and groups treated by DPM, ADO, DPM and theophyline (TH). Pulmonary pathology from day 4 to day 30 observations was studied by light and electron microscope. RESULTS:Alveolar epithelial cell necrosis, lung tissue lesions by hypoxia and interstitial fibrosis were found in the BLM group after day 10. The necrotic epithelial cell, fibroblast proliferation, and the hypoxia induced tissue damage were less prominent, and the number and phagocytic function of macrophages were increased in the DPM and ADO treated groups. In the TH treated group, necrotic epithelial cells increased, the lung tissue was ischemic, and the differentiation of monocytes in pulmonary interstitial was slower. CONCLUSION:DPM and ADO can inhibit fibroblast proliferation by improving microcirculation and promoting macrophage development and alveolar space reconstruction.