STUDY DESIGN: Herniated lumbar disc specimens were analyzed using reverse transcriptase-polymerase chain reaction to determine the profile of chemokine expression. OBJECTIVE: To investigate the mechanism underlying the recruitment of inflammatory cells into herniated discs during the process of spontaneous regression. SUMMARY OF BACKGROUND DATA: Spontaneous regression of herniated intervertebral discs has been increasingly reported. Although macrophages are suggested to play a central role in this process, it remains unclear how these macrophages accumulate in the herniated discs. METHODS: RNA was extracted from 36 surgical specimens of the herniated lumbar disc, a disc specimen of idiopathic scoliosis and pyogenic spondylitis, and activated peripheral blood mononuclear cells of a normal donor. The RNA was reverse transcribed, and the resultant cDNA was amplified by PCR using primer pairs specific to the CXC chemokines (IL-8, MGSA-alpha, IP-10, MIG), the CC chemokines (MCP-1, MCP-2, MCP-3, MCP-4, MIP-1alpha, MIP-3alpha, RANTES, STCP-1), the C chemokine (lymphotactin), and the glyceraldehyde phosphate housekeeping gene. Thin cryostat sections also were made from the disc specimens and stained with hematoxylin and eosin. RESULTS: All the chemokines examined except MCP-4 were expressed by activated peripheral blood mononuclear cells. Glyceraldehyde phosphate was detected in 8 of 36 herniated discs and in 1 disc specimen each of idiopathic scoliosis and pyogenic spondylitis. Chemokine expression was examined for these 10 disc specimens. From among the 13 chemokines examined, MCP-3, MCP-4, RANTES, and IP-10 were detected in the disc from the idiopathic scoliosis, and MCP-3, MCP-4, RANTES, IP-10, MIG, and MGSA-alpha were detected in the infected or herniated discs. Histologic analysis showed infiltration of inflammatory cells in the infected disc and all 8 herniated discs. CONCLUSIONS: The findings suggest that chemoattractive properties exist in a selected population of human intervertebral discs, and that unique sets of chemokinesplay a role in spontaneous regression of these herniated disc tissues.
STUDY DESIGN: Herniated lumbar disc specimens were analyzed using reverse transcriptase-polymerase chain reaction to determine the profile of chemokine expression. OBJECTIVE: To investigate the mechanism underlying the recruitment of inflammatory cells into herniated discs during the process of spontaneous regression. SUMMARY OF BACKGROUND DATA: Spontaneous regression of herniated intervertebral discs has been increasingly reported. Although macrophages are suggested to play a central role in this process, it remains unclear how these macrophages accumulate in the herniated discs. METHODS: RNA was extracted from 36 surgical specimens of the herniated lumbar disc, a disc specimen of idiopathic scoliosis and pyogenic spondylitis, and activated peripheral blood mononuclear cells of a normal donor. The RNA was reverse transcribed, and the resultant cDNA was amplified by PCR using primer pairs specific to the CXC chemokines (IL-8, MGSA-alpha, IP-10, MIG), the CC chemokines (MCP-1, MCP-2, MCP-3, MCP-4, MIP-1alpha, MIP-3alpha, RANTES, STCP-1), the C chemokine (lymphotactin), and the glyceraldehyde phosphate housekeeping gene. Thin cryostat sections also were made from the disc specimens and stained with hematoxylin and eosin. RESULTS: All the chemokines examined except MCP-4 were expressed by activated peripheral blood mononuclear cells. Glyceraldehyde phosphate was detected in 8 of 36 herniated discs and in 1 disc specimen each of idiopathic scoliosis and pyogenic spondylitis. Chemokine expression was examined for these 10 disc specimens. From among the 13 chemokines examined, MCP-3, MCP-4, RANTES, and IP-10 were detected in the disc from the idiopathic scoliosis, and MCP-3, MCP-4, RANTES, IP-10, MIG, and MGSA-alpha were detected in the infected or herniated discs. Histologic analysis showed infiltration of inflammatory cells in the infected disc and all 8 herniated discs. CONCLUSIONS: The findings suggest that chemoattractive properties exist in a selected population of human intervertebral discs, and that unique sets of chemokinesplay a role in spontaneous regression of these herniated disc tissues.
Authors: Jianru Wang; Ye Tian; Kate L E Phillips; Neil Chiverton; Gail Haddock; Rowena A Bunning; Alison K Cross; Irving M Shapiro; Christine L Le Maitre; Makarand V Risbud Journal: Arthritis Rheum Date: 2013-03
Authors: Min Ho Hwang; Dong Hyun Cho; Seung Min Baek; Jae Won Lee; Jeong Hun Park; Chang Min Yoo; Jae Hee Shin; Hyo Geun Nam; Hyeong Guk Son; Hyun Jung Lim; Han Sang Cho; Hong Joo Moon; Joo Han Kim; Jong Kwang Lee; Hyuk Choi Journal: Biomicrofluidics Date: 2017-12-06 Impact factor: 2.800
Authors: Min Ho Hwang; Kyoung Soo Kim; Chang Min Yoo; Jae Hee Shin; Hyo Geun Nam; Jin Su Jeong; Joo Han Kim; Kwang Ho Lee; Hyuk Choi Journal: Lasers Med Sci Date: 2016-03-17 Impact factor: 3.161
Authors: Emerson Krock; Derek H Rosenzweig; Anne-Julie Chabot-Doré; Peter Jarzem; Michael H Weber; Jean A Ouellet; Laura S Stone; Lisbet Haglund Journal: J Cell Mol Med Date: 2014-03-20 Impact factor: 5.310