OBJECTIVE: To clarify the influence of moderate hypothermia on the production of proinflammatory cytokines. DESIGN: Controlled in vitro study. SETTING: Research laboratory. SUBJECTS: Peripheral blood mononuclear cells from healthy adult human subjects. INTERVENTIONS: Stimulation with 1 microg/mL lipopolysaccharide at 33 degrees C and 37 degrees C. MEASUREMENTS: Concentrations of released tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 were measured chronologically by enzyme immunoassay. The number of mRNA copies of these cytokines was determined by competitive reverse transcriptase-polymerase chain reaction analysis, and nuclear factor-kappaB activations were assessed by electrophoretic mobility shift assay. MAIN RESULTS: Significant reduction of the released-tumor necrosis factor-alpha concentration was observed 1 and 2 hrs after the stimulation with lipopolysaccharide at 33 degrees C compared with 37 degrees C. The peak release of interleukin-1beta at 33 degrees C was delayed 12 hrs later than that at 37 degrees C. A delayed peak in the release of interleukin-6 also was observed at 33 degrees C. The peaks of cytokines were confirmed at the mRNA expression level by competitive reverse transcriptase-polymerase chain reaction analysis at both temperatures. The peak of the tumor necrosis factor-alpha mRNA expression level was observed at 1 hr after the stimulation at 37 degrees C and 2 hrs after the stimulation at 33 degrees C. In the interleukin-1beta mRNA expression, at 37 degrees C the first peak appeared 1 hr and the second 6 hrs after the stimulation. In contrast, at 33 degrees C, the first peak appeared 2 hrs and the second 12 hrs after the stimulation. Whereas interleukin-6 mRNA expression at 37 degrees C peaked 6 hrs after the stimulation, no definite peak was observed at 33 degrees C and the expression level was approximately half of that at 37 degrees C. The maximum intensity of nuclear factor-kappaB activation at 33 degrees C was delayed by 1.5 hrs compared with that at 37 degrees C. CONCLUSIONS: Moderate hypothermia delays the induction of proinflammatory cytokines in human peripheral blood mononuclear cells.
OBJECTIVE: To clarify the influence of moderate hypothermia on the production of proinflammatory cytokines. DESIGN: Controlled in vitro study. SETTING: Research laboratory. SUBJECTS: Peripheral blood mononuclear cells from healthy adult human subjects. INTERVENTIONS: Stimulation with 1 microg/mL lipopolysaccharide at 33 degrees C and 37 degrees C. MEASUREMENTS: Concentrations of released tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 were measured chronologically by enzyme immunoassay. The number of mRNA copies of these cytokines was determined by competitive reverse transcriptase-polymerase chain reaction analysis, and nuclear factor-kappaB activations were assessed by electrophoretic mobility shift assay. MAIN RESULTS: Significant reduction of the released-tumor necrosis factor-alpha concentration was observed 1 and 2 hrs after the stimulation with lipopolysaccharide at 33 degrees C compared with 37 degrees C. The peak release of interleukin-1beta at 33 degrees C was delayed 12 hrs later than that at 37 degrees C. A delayed peak in the release of interleukin-6 also was observed at 33 degrees C. The peaks of cytokines were confirmed at the mRNA expression level by competitive reverse transcriptase-polymerase chain reaction analysis at both temperatures. The peak of the tumor necrosis factor-alpha mRNA expression level was observed at 1 hr after the stimulation at 37 degrees C and 2 hrs after the stimulation at 33 degrees C. In the interleukin-1beta mRNA expression, at 37 degrees C the first peak appeared 1 hr and the second 6 hrs after the stimulation. In contrast, at 33 degrees C, the first peak appeared 2 hrs and the second 12 hrs after the stimulation. Whereas interleukin-6 mRNA expression at 37 degrees C peaked 6 hrs after the stimulation, no definite peak was observed at 33 degrees C and the expression level was approximately half of that at 37 degrees C. The maximum intensity of nuclear factor-kappaB activation at 33 degrees C was delayed by 1.5 hrs compared with that at 37 degrees C. CONCLUSIONS: Moderate hypothermia delays the induction of proinflammatory cytokines in human peripheral blood mononuclear cells.
Authors: M M Ning; M Lopez; D Sarracino; J Cao; M Karchin; D McMullin; X Wang; F S Buonanno; E H Lo Journal: Neurol Res Date: 2013-06 Impact factor: 2.448