Literature DB >> 121261

[Determination of free intracellular proline pool in fibroblast cultures].

A Chamson, C Perier, A Rattner, J Frey.   

Abstract

By using a direct, rapid, sensitive colorimetric method, free intracellular proline pool from human adult skin fibroblasts in monolayer was determined. Thus under identical experimental conditions, free intracellular proline pool was about 20 pmol proline/micrograms cellular proteins, whatever the cell lines from human adult skin fibroblasts, the passage and the time of subculture. Besides, there was a poor correlation between collagen biosynthesis in fibroblast cultures and free intracellular proline pool.

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Year:  1979        PMID: 121261

Source DB:  PubMed          Journal:  C R Seances Acad Sci D        ISSN: 0567-655X


  1 in total

1.  In-vitro responses to ascorbate and manganese in fibroblasts from a patient with prolidase deficiency and iminodipeptiduria: cell growth, prolidase activity and collagen metabolism.

Authors:  I Myara; C Charpentier; C Wolfrom; M Gautier; A Lemonnier; M Larregue; A Chamson; J Frey
Journal:  J Inherit Metab Dis       Date:  1983       Impact factor: 4.982

  1 in total

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