Detection of reactive oxygen and nitrogen species in tissues using redox-sensitive fluorescent probes.

The take-home message of this chapter is that the fluorescent probes for ROS and RNS have great potential in improving our understanding of redox behavior within cells and tissues. However, data obtained from studies using these probes must be expressed in the context of the limitations of the chemistry of the probes in the cellular microenvironment, which may change under different conditions, such as cell stress or injury. In most cases, as suggested, results should be described in a general context of reflecting an increase in oxidizing reactions within the cell and not as a quantitative measure of the production of a specific oxidant species. It is highly recommended that results be verified, when possible, with alternative fluorescent probes or preferably using alternative methods, such as electron spin resonance or other newly emerging technology.