Literature DB >> 121242

The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations.

K Nielsen, B S Samagh, G Speckmann, B Stemshorn.   

Abstract

The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattle sera were eliminated while no decrease in titer was apparent when sera from B. abortus infected or vaccinated cattle were tested.

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Year:  1979        PMID： 121242      PMCID： PMC1320015     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN： 0008-4050


  16 in total

1.  A heat-inactivation test for differentiating specific and non-specific agglutination reactions for bovine brucellosis.

Authors:  T E AMERAULT; C A MANTHEI; E R GOODE; G LAMBERT
Journal:  Am J Vet Res       Date:  1961-05       Impact factor: 1.156

2.  An acidified antigen for detection of nonspecific reactions in the plate-agglutination test for bovine brucellosis.

Authors:  J E ROSE; M H ROEPKE
Journal:  Am J Vet Res       Date:  1957-07       Impact factor: 1.156

3.  Studies on a nonspecific Brucella-agglutinating substance in bovin serum. I. The differentiation of the specific and nonspecific agglutinins by heat treatment.

Authors:  W R HESS
Journal:  Am J Vet Res       Date:  1953-04       Impact factor: 1.156

4.  Studies on a nonspecific Brucella-agglutinating substance in bovine serum. II. Isolation and purification of the Brucella-agglutinating substances.

Authors:  W R HESS
Journal:  Am J Vet Res       Date:  1953-04       Impact factor: 1.156

5.  Mechanisms of prozone formation in agglutination reaction.

Authors:  H J Cho; D G Ingram
Journal:  Can J Microbiol       Date:  1972-04       Impact factor: 2.419

6.  [Specificity limits in the brucellosis slow agglutination test].

Authors:  E Scheibner
Journal:  Berl Munch Tierarztl Wochenschr       Date:  1976-08-01       Impact factor: 0.328

7.  Differentiation of cross-reacting antibodies against brucella abortus and yersinia enterocolitica by electroimmuno assay.

Authors:  B Hurvell
Journal:  Acta Vet Scand       Date:  1975       Impact factor: 1.695

8.  Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection.

Authors:  G G Schurig; L M Jones; S L Speth; D T Berman
Journal:  Infect Immun       Date:  1978-09       Impact factor: 3.441

9.  The bovine immune response to Brucella abortus I. A water soluble antigen precipitated by sera of some naturally infected cattle.

Authors:  B Stemshorn; K Nielsen
Journal:  Can J Comp Med       Date:  1977-04

10.  Ultracentrifugal studies of sera from cattle vaccinated or naturally infected with Brucella abortus.

Authors:  C E Rice; J Tailyour; D Cochrane
Journal:  Can J Comp Med Vet Sci       Date:  1966-10
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  4 in total

1.  An investigation of the etiology of Brucella abortus singleton reactors.

Authors:  T W Dukes; K H Nielsen; M D Eaglesome; G W Speckmann; A H Corner
Journal:  Can J Comp Med       Date:  1980-10

2.  The use of divalent cation chelating agents (EDTA/EGTA) to reduce non-specific serum protein interaction in enzyme immunoassay.

Authors:  K Nielsen; L Kelly; D Gall; P Smith; J Bosse; P Nicoletti; W Kelly
Journal:  Vet Res Commun       Date:  1994       Impact factor: 2.459

3.  Ethylenediaminetetraacetic acid (disodium salt)-labile bovine immunoglobulin M Fc binding to Brucella abortus: a cause of nonspecific agglutination.

Authors:  K Nielsen; K Stilwell; B Stemshorn; R Duncan
Journal:  J Clin Microbiol       Date:  1981-07       Impact factor: 5.948

4.  The bovine immune response to Brucella abortus. III. Preparation of antisera against a Brucella component precipitated by sera of some infected cattle.

Authors:  B Stemshorn; K Nielsen; B Samagh
Journal:  Can J Comp Med       Date:  1981-01
  4 in total

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