Literature DB >> 12123677

Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations.

Sebastiana Angelaccio1, Maria Carmela Bonaccorsi di Patti.   

Abstract

Mesh:

Substances:

Year:  2002        PMID: 12123677     DOI: 10.1006/abio.2002.5689

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


× No keyword cloud information.
  13 in total

1.  Multiple site directed mutagenesis strategy based on total RNA and RT-PCR method.

Authors:  Zsolt B Nagy; Márk Gárdonyi; Attila Mészáros; Zoltán Varga-Orvos; Robert G Solomon; László Tamás
Journal:  Mol Biotechnol       Date:  2007-07-19       Impact factor: 2.695

2.  Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.

Authors:  David L Steffens; John G K Williams
Journal:  J Biomol Tech       Date:  2007-07

3.  Long distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers.

Authors:  Yuling Bu; Hui Wang; Jing Li; Zhaoxin Zhang; Yinghe Hu; Zhiliang Xu
Journal:  Mol Biotechnol       Date:  2013-09       Impact factor: 2.695

4.  Engineering of phenylacetaldehyde reductase for efficient substrate conversion in concentrated 2-propanol.

Authors:  Yoshihide Makino; Kousuke Inoue; Tohru Dairi; Nobuya Itoh
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

5.  Evolution and function of the Mycoplasma hyopneumoniae peroxiredoxin, a 2-Cys-like enzyme with a single Cys residue.

Authors:  Taylor Gonchoroski; Veridiana G Virginio; Claudia E Thompson; Jéssica A Paes; Cláudio X Machado; Henrique B Ferreira
Journal:  Mol Genet Genomics       Date:  2016-11-17       Impact factor: 3.291

6.  Improved activity of β-cyclodextrin glycosyltransferase from Bacillus sp. N-227 via mutagenesis of the conserved residues.

Authors:  Hua Wang; Wenxi Zhou; Hua Li; Bu Rie; Chunhong Piao
Journal:  3 Biotech       Date:  2017-06-08       Impact factor: 2.406

7.  Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold.

Authors:  Leif J Pallesen; Natalia Solodovnikova; Anil K Sharma; William E Walden
Journal:  J Biol Chem       Date:  2013-06-24       Impact factor: 5.157

8.  Megaprimer-based methodology for deletion of a large fragment within a repetitive polypyrimidine-rich DNA.

Authors:  Malin Söderberg; Matti A Lang
Journal:  Mol Biotechnol       Date:  2006-01       Impact factor: 2.695

9.  Simultaneous amplification, detection, and analysis of common mutations in the galactose-1-phosphate uridyl transferase gene.

Authors:  Mohamed Jama; Lesa Nelson; Genevieve Pont-Kingdon; Rong Mao; Elaine Lyon
Journal:  J Mol Diagn       Date:  2007-09-20       Impact factor: 5.568

10.  Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex.

Authors:  Haresh T More; Joseph A Frezzo; Jisen Dai; Seiichi Yamano; Jin K Montclare
Journal:  Biomaterials       Date:  2014-05-27       Impact factor: 12.479

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.