| Literature DB >> 12122430 |
Bartel Vanholme1, Jan De Meutter, Tom Tytgat, Greetje D C Gheysen, Isabelle Vanhoutte, Godelieve D R Gheysen.
Abstract
An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.Entities:
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Year: 2002 PMID： 12122430 DOI： 10.1007/s00436-002-0661-0
Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289