OBJECTIVES: This study was conducted to examine the memory T-cell response to dengue virus 20 years after a primary infection. We took advantage of the exceptional epidemiologic situation in Cuba, where the population initially suffered two large successive epidemics due to dengue virus 1 and 2 respectively over a 4-year period. Thereafter, no dengue virus circulation was subsequently observed, except for the Santiago de Cuba municipality. DESIGN: T-cell response was evaluated in peripheral blood mononuclear cells (PBMCs) from 20 individuals with history of a primary infection by dengue virus 1 or 2. Methods previously shown to induce lymphoproliferation of CD4+ memory T-cell subpopulations were used. We evaluated the proliferative responses generated in those PBMCs after stimulation with dengue virus 1, 2, 3 and 4 antigens in a serotype-specific and serotype-crossreactive way. RESULTS: Serotype-specific and serotype-crossreactive lymphoproliferative responses in all PBMCs donated by dengue immune donors were observed. The serotype-crossreactive response for dengue 2 was stronger than for the rest of the serotypes. CONCLUSIONS: This is the first report of cellular memory lymphocyte response specific for dengue virus detected 20 years after a primary infection by dengue.
OBJECTIVES: This study was conducted to examine the memory T-cell response to dengue virus 20 years after a primary infection. We took advantage of the exceptional epidemiologic situation in Cuba, where the population initially suffered two large successive epidemics due to dengue virus 1 and 2 respectively over a 4-year period. Thereafter, no dengue virus circulation was subsequently observed, except for the Santiago de Cuba municipality. DESIGN: T-cell response was evaluated in peripheral blood mononuclear cells (PBMCs) from 20 individuals with history of a primary infection by dengue virus 1 or 2. Methods previously shown to induce lymphoproliferation of CD4+ memory T-cell subpopulations were used. We evaluated the proliferative responses generated in those PBMCs after stimulation with dengue virus 1, 2, 3 and 4 antigens in a serotype-specific and serotype-crossreactive way. RESULTS: Serotype-specific and serotype-crossreactive lymphoproliferative responses in all PBMCs donated by dengue immune donors were observed. The serotype-crossreactive response for dengue 2 was stronger than for the rest of the serotypes. CONCLUSIONS: This is the first report of cellular memory lymphocyte response specific for dengue virus detected 20 years after a primary infection by dengue.
Authors: Stefan Fernandez; Emily D Cisney; Alexander P Tikhonov; Barry Schweitzer; Robert J Putnak; Monika Simmons; Robert G Ulrich Journal: Clin Vaccine Immunol Date: 2011-01-26
Authors: Scott R Fry; Michelle Meyer; Matthew G Semple; Cameron P Simmons; Shamala Devi Sekaran; Johnny X Huang; Catriona McElnea; Chang-Yi Huang; Andrea Valks; Paul R Young; Matthew A Cooper Journal: PLoS Negl Trop Dis Date: 2011-06-21
Authors: Heather Friberg; Hema Bashyam; Tomoko Toyosaki-Maeda; James A Potts; Thomas Greenough; Siripen Kalayanarooj; Robert V Gibbons; Ananda Nisalak; Anon Srikiatkhachorn; Sharone Green; Henry A F Stephens; Alan L Rothman; Anuja Mathew Journal: Sci Rep Date: 2011-08-01 Impact factor: 4.379