Visible light induced biohydrogen production from sucrose using the photosensitization of Mg chlorophyll-a.

A photoinduced hydrogen production system, coupling sucrose degradation with invertase and glucose dehydrogenase (GDH) and hydrogen production with colloidal platinum as a catalyst using the visible light-induced photosensitization of Mg chlorophyll-a (Mg Chl-a), has been developed. Continuous hydrogen gas production was observed when the reaction mixture containing sucrose, invertase, GDH, nicotinamide adenine dinucleotide (NAD(+)), Mg Chl-a, methyl viologen (MV(2+), an electron relay reagent), and colloidal platinum was irradiated by visible light.