| Literature DB >> 12116131 |
Otto Bekers1, Robert A M op den Buijsch, Johan E de Vries, Petal A H M Wijnen, Marja P van Dieijen-Visser.
Abstract
We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20-100 base pairs. Especially discrimination between apoE2/E2 and apoE2/E3 genotypes is more reliable with CE than with AGE, this being of great clinical value in the diagnosis of familiary dysbetalipoproteinemia.Entities:
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Year: 2002 PMID： 12116131 DOI： 10.1002/1522-2683(200206)23:12<1878::AID-ELPS1878>3.0.CO;2-G
Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535