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Literature DB >> 12113822

Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases.

Sang Kook Kim¹, Ji Young Kim, Ae Kyeong Baek, Byung Jo Moon.

Abstract

Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs with thymine and guanine with similar stabilities.

Entities: Chemical

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Year: 2002 PMID： 12113822 DOI： 10.1016/s0960-894x(02)00297-4

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

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Cited

6 in total

1. Synthesis, base pairing properties and trans-lesion synthesis by reverse transcriptases of oligoribonucleotides containing the oxidatively damaged base 5-hydroxycytidine.

Authors: Pascal A Küpfer; Christian J Leumann
Journal: Nucleic Acids Res Date: 2011-08-18 Impact factor: 16.971

6. Experimental and theoretical rationalization for the base pairing abilities of inosine, guanosine, adenosine, and their corresponding 8-oxo-7,8-dihydropurine, and 8-bromopurine analogues within A-form duplexes of RNA.

Authors: Austin Skinner; Chou-Hsun Yang; Kazuki Hincks; Haobin Wang; Marino J E Resendiz
Journal: Biopolymers Date: 2020-11-20 Impact factor: 2.505

6 in total

Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases.

1. Synthesis, base pairing properties and trans-lesion synthesis by reverse transcriptases of oligoribonucleotides containing the oxidatively damaged base 5-hydroxycytidine.

2. Biophysical properties and thermal stability of oligonucleotides of RNA containing 7,8-dihydro-8-hydroxyadenosine.

3. The effect of RNA base lesions on mRNA translation.

4. Oxidative stress damages rRNA inside the ribosome and differentially affects the catalytic center.

5. Structure-Activity Relationships Reveal Key Features of 8-Oxoguanine: A Mismatch Detection by the MutY Glycosylase.

6. Experimental and theoretical rationalization for the base pairing abilities of inosine, guanosine, adenosine, and their corresponding 8-oxo-7,8-dihydropurine, and 8-bromopurine analogues within A-form duplexes of RNA.