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Literature DB >> 121120

Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography.

Abstract

A sensitive method for the determination of chondroitin 4- and 6-sulphate is presented. After chondroitinase digestion of the chondroitin sulphate preparations, the obtained disaccharides are separated on a weak anion-exchange resin in a high-performance liquid chromatography system. The method was used to study 4-sulphate to 6-sulphate ratios in chondroitin sulphates prepared from bovine nasal cartilage and human nucleus pulposus. The results show clearly that these two preparations contain considerable amounts of both isomers.

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Year: 1979 PMID： 121120 DOI： 10.1016/s0021-9673(01)95313-0

Source DB: PubMed Journal: J Chromatogr

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Cited

17 in total

1. Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1. Induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163-171.

Authors: A E Postlethwaite; G N Smith; L B Lachman; R O Endres; H M Poppleton; K A Hasty; J M Seyer; A H Kang
Journal: J Clin Invest Date: 1989-02 Impact factor: 14.808

2. Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.

Authors: C H Zheng; M E Levenston
Journal: Eur Cell Mater Date: 2015-04-19 Impact factor: 3.942

3. Isolation and characterization of proteoglycans from human follicular fluid.

Authors: G V Eriksen; I Carlstedt; M Mörgelin; N Uldbjerg; A Malmström
Journal: Biochem J Date: 1999-06-15 Impact factor: 3.857

4. Bovine sesamoid bones: a culture system for anatomically intact articular cartilage.

Authors: G H Korver; R J van de Stadt; G P van Kampen; E Kiljan; J K van der Korst
Journal: In Vitro Cell Dev Biol Date: 1989-12

Review 5. On-line separations combined with MS for analysis of glycosaminoglycans.

Authors: Joseph Zaia
Journal: Mass Spectrom Rev Date: 2009 Mar-Apr Impact factor: 10.946

6. The growth promoter spermine interacts specifically with dermatan sulfate regions that are rich in L-iduronic acid and possess antiproliferative activity.

Authors: M Belting; L A Fransson
Journal: Glycoconj J Date: 1993-12 Impact factor: 2.916

Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography.

1. Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1. Induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163-171.

2. Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.

3. Isolation and characterization of proteoglycans from human follicular fluid.

4. Bovine sesamoid bones: a culture system for anatomically intact articular cartilage.

Review 5. On-line separations combined with MS for analysis of glycosaminoglycans.

6. The growth promoter spermine interacts specifically with dermatan sulfate regions that are rich in L-iduronic acid and possess antiproliferative activity.

7. Isolation and characterization of high-buoyant-density proteoglycans from bovine femoral-head cartilage.

8. Isolation and partial characterization of proteoglycans from rat incisors.

9. Analysis of dentine glycosaminoglycans using high-performance liquid chromatography.

10. Metabolism of rat bone proteoglycans in vivo.