Literature DB >> 1210987

[Rapid treatment of nervous tissue for the purpose of electron microscopy].

V A Agafonov.   

Abstract

The paper deals with the description of the method which permits to prepare during 3.5 hours the nervous tissue for an electrono-microscopic study. The accelarated processing is achieved due to the reduction of the main stages: prefixation, fixation, dehydration, eponic saturation and polymerization. The isotonic buffer was used for the removal of a possible deformation of cell appendexes (pressing) with the fixation in glutaraldehyde. The accelarated method of the nervous tissue processing neither gives afrtefacts, nor reveals essential differences in the tissue in comparison with the general method.

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Year:  1975        PMID: 1210987

Source DB:  PubMed          Journal:  Zh Nevropatol Psikhiatr Im S S Korsakova        ISSN: 0044-4588


  1 in total

1.  Comparative electron microscopic and autoradiographic studies of "dark" and "light" neurons of the cerebral cortex.

Authors:  E Y Rubleva; Y I Savulev; A S Pylaev
Journal:  Neurosci Behav Physiol       Date:  1980 Jan-Feb
  1 in total

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