Literature DB >> 12107154

Mutant analysis shows that alanine racemases from Pseudomonas aeruginosa and Escherichia coli are dimeric.

Ulrich Strych1, Michael J Benedik.   

Abstract

Alanine racemases are ubiquitous prokaryotic enzymes providing the essential peptidoglycan precursor D-alanine. We present evidence that the enzymes from Pseudomonas aeruginosa and Escherichia coli function exclusively as homodimers. Moreover, we demonstrate that expression of a K35A Y235A double mutation of dadX in E. coli suppresses bacterial growth in a dominant negative fashion.

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Year:  2002        PMID： 12107154      PMCID： PMC135199          DOI： 10.1128/JB.184.15.4321-4325.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Role of lysine 39 of alanine racemase from Bacillus stearothermophilus that binds pyridoxal 5'-phosphate. Chemical rescue studies of Lys39 --> Ala mutant.

Authors:  A Watanabe; Y Kurokawa; T Yoshimura; T Kurihara; K Soda; N Esaki; A Watababe
Journal:  J Biol Chem       Date:  1999-02-12       Impact factor: 5.157

2.  Purification and some properties of alanine racemase from a bivalve mollusc Corbicula japonica.

Authors:  T Nomura; I Yamamoto; F Morishita; Y Furukawa; O Matsushima
Journal:  J Exp Zool       Date:  2001-01-01

3.  Biosynthetic alanine racemase of Salmonella typhimurium: purification and characterization of the enzyme encoded by the alr gene.

Authors:  N Esaki; C T Walsh
Journal:  Biochemistry       Date:  1986-06-03       Impact factor: 3.162

4.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.

Authors:  U Strych; H C Huang; K L Krause; M J Benedik
Journal:  Curr Microbiol       Date:  2000-10       Impact factor: 2.188

6.  Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution.

Authors:  J P Shaw; G A Petsko; D Ringe
Journal:  Biochemistry       Date:  1997-02-11       Impact factor: 3.162

7.  Structure of a Michaelis complex analogue: propionate binds in the substrate carboxylate site of alanine racemase.

Authors:  A A Morollo; G A Petsko; D Ringe
Journal:  Biochemistry       Date:  1999-03-16       Impact factor: 3.162

8.  Gene cloning and characterization of alanine racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei.

Authors:  K Yokoigawa; R Hirasawa; H Ueno; Y Okubo; S Umesako; K Soda
Journal:  Biochem Biophys Res Commun       Date:  2001-11-02       Impact factor: 3.575

9.  Reaction of alanine racemase with 1-aminoethylphosphonic acid forms a stable external aldimine.

Authors:  G F Stamper; A A Morollo; D Ringe; C G Stamper
Journal:  Biochemistry       Date:  1998-07-21       Impact factor: 3.162

10.  Catabolic alanine racemase from Salmonella typhimurium: DNA sequence, enzyme purification, and characterization.

Authors:  S A Wasserman; E Daub; P Grisafi; D Botstein; C T Walsh
Journal:  Biochemistry       Date:  1984-10-23       Impact factor: 3.162
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  14 in total

1.  Membrane fusion during phage lysis.

Authors:  Manoj Rajaure; Joel Berry; Rohit Kongari; Jesse Cahill; Ry Young
Journal:  Proc Natl Acad Sci U S A       Date:  2015-04-13       Impact factor: 11.205

2.  Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.

Authors:  Honggang Zhou; Zhenzhen Li; Guofang Zhang; Shujing Xu; Zhaona Tang; Xianming Zhu; Hui Dong; Jiansong Ju
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-11-14       Impact factor: 1.056

3.  Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26.

Authors:  Jun-Lin Liu; Xiao-Qin Liu; Ya-Wei Shi
Journal:  World J Microbiol Biotechnol       Date:  2011-06-21       Impact factor: 3.312

4.  Structural basis for the broad specificity of a new family of amino-acid racemases.

Authors:  Akbar Espaillat; César Carrasco-López; Noelia Bernardo-García; Natalia Pietrosemoli; Lisandro H Otero; Laura Álvarez; Miguel A de Pedro; Florencio Pazos; Brigid M Davis; Matthew K Waldor; Juan A Hermoso; Felipe Cava
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2013-12-24

5.  Listeria monocytogenes-based antibiotic resistance gene-free antigen delivery system applicable to other bacterial vectors and DNA vaccines.

Authors:  Thorsten Verch; Zhen-Kun Pan; Yvonne Paterson
Journal:  Infect Immun       Date:  2004-11       Impact factor: 3.441

6.  A structure-based design approach for the identification of novel inhibitors: application to an alanine racemase.

Authors:  Gabriela Iurcu Mustata; James M Briggs
Journal:  J Comput Aided Mol Des       Date:  2002-12       Impact factor: 3.686

7.  The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design.

Authors:  Hookang Im; Miriam L Sharpe; Ulrich Strych; Milya Davlieva; Kurt L Krause
Journal:  BMC Microbiol       Date:  2011-05-25       Impact factor: 3.605

8.  Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker.

Authors:  Sheryl L W Zajdowicz; Jessica Jones-Carson; Andres Vazquez-Torres; Michael G Jobling; Ronald E Gill; Randall K Holmes
Journal:  PLoS One       Date:  2011-06-24       Impact factor: 3.240

9.  Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50).

Authors:  Emma R Scaletti; Sylvia R Luckner; Kurt L Krause
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-12-09

10.  Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames).

Authors:  Rafael M Couñago; Milya Davlieva; Ulrich Strych; Ryan E Hill; Kurt L Krause
Journal:  BMC Struct Biol       Date:  2009-08-20
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