AIM: To examine the effect of mycophenolic acid (MPA) on the activity of nuclear factor-kappaB (NF-kappaB) and its inhibitor (IkappaBalpha) in 4-phorbal-12-myristate-13-acetate (PMA) stimulated and non-stimulated human umbilical vein endothelial cells (HUVEC). METHODS: MPA at various concentrations were applied to cultured HUVEC, pre-incubated or not pre-incubated with PMA (100 microg/L for 30 min). The activity of NF-kappaB in endothelial cells was measured with electrophoretic mobility shift assays (EMSA), and IkappaBalpha protein levels were detected by Western blot method. RESULTS: Incubation with PMA was found to result in rapid increment of NF-kappaB activity in cultured HUVEC. MPA treatment decreased the activities of NF-kappaB, both in PMA treatment and un-treatment HUVEC. Protein level of IkappaBalpha was decreased in PMA-treatment HUVEC. MPA treatment increased IkappaBalpha protein levels in both PMA-treatment and un-treatment HUVEC. CONCLUSION: MPA is a potent inhibitor of NF-kappaB activation in endothelial cells, which might explain its beneficial effect in endothelial cells.
AIM: To examine the effect of mycophenolic acid (MPA) on the activity of nuclear factor-kappaB (NF-kappaB) and its inhibitor (IkappaBalpha) in 4-phorbal-12-myristate-13-acetate (PMA) stimulated and non-stimulated human umbilical vein endothelial cells (HUVEC). METHODS:MPA at various concentrations were applied to cultured HUVEC, pre-incubated or not pre-incubated with PMA (100 microg/L for 30 min). The activity of NF-kappaB in endothelial cells was measured with electrophoretic mobility shift assays (EMSA), and IkappaBalpha protein levels were detected by Western blot method. RESULTS: Incubation with PMA was found to result in rapid increment of NF-kappaB activity in cultured HUVEC. MPA treatment decreased the activities of NF-kappaB, both in PMA treatment and un-treatment HUVEC. Protein level of IkappaBalpha was decreased in PMA-treatment HUVEC. MPA treatment increased IkappaBalpha protein levels in both PMA-treatment and un-treatment HUVEC. CONCLUSION:MPA is a potent inhibitor of NF-kappaB activation in endothelial cells, which might explain its beneficial effect in endothelial cells.
Authors: Christophe Richez; Rocco J Richards; Pierre Duffau; Zachary Weitzner; Christopher D Andry; Ian R Rifkin; Tamar Aprahamian Journal: PLoS One Date: 2013-04-08 Impact factor: 3.240