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Literature DB >> 12099271

Glufosinate-tolerant tobacco plants directed by the promoter of adenylate kinase gene of rice.

Hiromitsu Fukuzawa¹, Satoshi Arai, Maki Kawai-Yamada, Avijit Das, Michito Tagawa, Hirofumi Uchimiya.

Abstract

A DNA clone containing the 5' part of the adenylate kinase (AK) gene was isolated from a rice genomic library, and its nucleotide sequence was determined. This clone consists of 5' upstream, five exons and four introns of the AK gene. All of the determined donor and receptor sites contained 'GT' and 'AG' consensus splice sequences. Transgenic tobacco plants harbouring a chimeric gene consisting of the 5' upstream sequence of the AK gene fused with the gene encoding phosphinothricin acetyl transferase were generated. They showed tolerance to glufosinate to a level four times higher than its commercial dose.

Entities: Chemical Gene Species

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Year: 2002 PMID： 12099271 PMCID： PMC4233820 DOI： 10.1093/aob/mcf041

Source DB: PubMed Journal: Ann Bot ISSN： 0305-7364 Impact factor: 4.357

9 in total

1 in total

1. The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm.

Authors: Hiromitsu Fukuzawa; Li-Hua Yu; Chikage Umeda-Hara; Michito Tagawa; Hirofumi Uchimiya
Journal: Plant Cell Rep Date: 2004-07-20 Impact factor: 4.570

1 in total

Glufosinate-tolerant tobacco plants directed by the promoter of adenylate kinase gene of rice.

1. Adenylate kinase from Pseudomonas denitrificans. I. Purification and antiserum inhibition.

2. The amino-acid sequence of sarcine adenylate kinase from skeletal muscle.

3. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.

4. Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants.

5. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids.

6. ATP:AMP phosphotransferase from baker's yeast. Purification and properties.

7. Biochemical properties of rice adenylate kinase and subcellular location in plant cells.

8. Molecular characterization of cDNA encoding for adenylate kinase of rice (Oryza sativa L.).

9. A simple and general method for transferring genes into plants.

1. The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm.