PURPOSE: The purpose of this study was to determine the metabolic, histologic, and ultrastructural effects of morphine and its combination with saline and bupivacaine on human articular cartilage. TYPE OF STUDY: In vitro study. METHODS: Nonfibrillated human articular cartilage was harvested and transferred into an experimental culture consisting of a control medium, saline, or a combination of morphine/saline or morphine/saline/bupivacaine for 12, 24, or 72 hours. Each sample was radiolabeled to assess proteoglycan synthesis. Histologic and ultrastructural effects were also examined. RESULTS: We found a significant, dose-related, transient decrease in 35SO4 incorporation in the morphine/saline samples at 12 hours, and in the saline only samples at 24 hours. We found no evidence of histologic or ultrastructural damage to the cartilage. CONCLUSIONS: Morphine and saline can both produce a transient decrease in 35SO4 incorporation that normalizes by 72 hours. This study does not suggest any contraindication to the use of intra-articular morphine as a postoperative analgesic.
PURPOSE: The purpose of this study was to determine the metabolic, histologic, and ultrastructural effects of morphine and its combination with saline and bupivacaine on humanarticular cartilage. TYPE OF STUDY: In vitro study. METHODS: Nonfibrillated humanarticular cartilage was harvested and transferred into an experimental culture consisting of a control medium, saline, or a combination of morphine/saline or morphine/saline/bupivacaine for 12, 24, or 72 hours. Each sample was radiolabeled to assess proteoglycan synthesis. Histologic and ultrastructural effects were also examined. RESULTS: We found a significant, dose-related, transient decrease in 35SO4 incorporation in the morphine/saline samples at 12 hours, and in the saline only samples at 24 hours. We found no evidence of histologic or ultrastructural damage to the cartilage. CONCLUSIONS:Morphine and saline can both produce a transient decrease in 35SO4 incorporation that normalizes by 72 hours. This study does not suggest any contraindication to the use of intra-articular morphine as a postoperative analgesic.
Authors: Florian Haasters; Hans Polzer; Wolf Christian Prall; Maximilian Michael Saller; Julia Kohler; Stefan Grote; Wolf Mutschler; Denitsa Docheva; Matthias Schieker Journal: Knee Surg Sports Traumatol Arthrosc Date: 2011-06-07 Impact factor: 4.342
Authors: Yi-lun Wang; Chao Zeng; Dong-xing Xie; Ye Yang; Jie Wei; Tuo Yang; Hui Li; Guang-hua Lei Journal: BMJ Open Date: 2015-06-15 Impact factor: 2.692
Authors: Charles J Cogan; Michael Knesek; Vehniah K Tjong; Rueben Nair; Cynthia Kahlenberg; Kevin F Dunne; Mark C Kendall; Michael A Terry Journal: Orthop J Sports Med Date: 2016-02-26