Literature DB >> 12096096

Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos.

Miho Ikeda-Iwai1, Shinobu Satoh, Hiroshi Kamada.   

Abstract

Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.

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Year:  2002        PMID： 12096096     DOI： 10.1093/jxb/erf006

Source DB:  PubMed          Journal:  J Exp Bot        ISSN： 0022-0957            Impact factor:   6.992


  25 in total

1.  Expression and maintenance of embryogenic potential is enhanced through constitutive expression of AGAMOUS-Like 15.

Authors:  Ellen W Harding; Weining Tang; Karl W Nichols; Donna E Fernandez; Sharyn E Perry
Journal:  Plant Physiol       Date:  2003-09-25       Impact factor: 8.340

2.  Isolation and characterization of differentially expressed transcripts from the suspension cells of oil palm (Elaeis guineensis Jacq.) in response to different concentration of auxins.

Authors:  Siti Habsah Roowi; Chai-Ling Ho; Sharifah Shahrul Rabiah Syed Alwee; Meilina Ong Abdullah; Suhaimi Napis
Journal:  Mol Biotechnol       Date:  2010-09       Impact factor: 2.695

3.  Molecular aspects of somatic-to-embryogenic transition in plants.

Authors:  Omid Karami; Behzad Aghavaisi; Aghil Mahmoudi Pour
Journal:  J Chem Biol       Date:  2009-09-10

4.  Establishment of a simple and efficient system for somatic embryo induction via ovule culture in Arabidopsis thaliana.

Authors:  Jun Wei; Xin-Ran Li; Meng-Xiang Sun
Journal:  Plant Cell Rep       Date:  2006-07-08       Impact factor: 4.570

5.  Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by orthologs of Agamous-like15 and Agamous-like18.

Authors:  Qiaolin Zheng; Sharyn E Perry
Journal:  Plant Physiol       Date:  2014-01-30       Impact factor: 8.340

6.  Transcript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus.

Authors:  Meghna R Malik; Feng Wang; Joan M Dirpaul; Ning Zhou; Patricia L Polowick; Alison M R Ferrie; Joan E Krochko
Journal:  Plant Physiol       Date:  2007-03-23       Impact factor: 8.340

7.  Auxin and root initiation in somatic embryos of Arabidopsis.

Authors:  Burgund M Bassuner; Russell Lam; Wolfgang Lukowitz; Edward C Yeung
Journal:  Plant Cell Rep       Date:  2006-07-25       Impact factor: 4.570

8.  Evolution of asexual reproduction in leaves of the genus Kalanchoë.

Authors:  Helena M P Garcês; Connie E M Champagne; Brad T Townsley; Soomin Park; Rui Malhó; Maria C Pedroso; John J Harada; Neelima R Sinha
Journal:  Proc Natl Acad Sci U S A       Date:  2007-09-24       Impact factor: 11.205

9.  The MADS-domain transcriptional regulator AGAMOUS-LIKE15 promotes somatic embryo development in Arabidopsis and soybean.

Authors:  Dhiraj Thakare; Weining Tang; Kristine Hill; Sharyn E Perry
Journal:  Plant Physiol       Date:  2008-02-27       Impact factor: 8.340

10.  Auxin-induced WUS expression is essential for embryonic stem cell renewal during somatic embryogenesis in Arabidopsis.

Authors:  Ying H Su; Xiang Y Zhao; Yu B Liu; Chuan L Zhang; Sharman D O'Neill; Xian S Zhang
Journal:  Plant J       Date:  2009-08       Impact factor: 6.417
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