Literature DB >> 12095275

Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography.

B H Ramsahoye1.   

Abstract

A method to determine the extent of cytosine methylation in DNA is described. The technique involves the enzymatic hydrolysis of DNA to its deoxyribonucleotide components and subsequent separation and quantification of the nucleotides by isocratic reversed-phase high-performance liquid chromatography (HPLC). The system gives highly reproducible results and, under suitable conditions, is capable of measuring 5-methylcytosine levels in as little as 1 microg of DNA. (c) 2002 Elsevier Science (USA).

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Year:  2002        PMID： 12095275     DOI： 10.1016/s1046-2023(02)00069-5

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  22 in total

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