Literature DB >> 12092840

Method for purification of fluorescence-labeled oligosaccharides by pyridylamination.

Shunji Natsuka1, Jiro Adachi, Masahumi Kawaguchi, Akira Ichikawa, Koji Ikura.   

Abstract

We developed a convenient method for purification of PA-oligosaccharides to remove contaminants originating from natural fluorescent materials, and excess reagents as well as by-products of tagging reactions in glycan analysis. The method, using a C18-cartridge, is simple and powerful to remove them. Several examples of experiments that showed the usefulness of this purification method are described in this report.

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Year:  2002        PMID： 12092840     DOI： 10.1271/bbb.66.1174

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  4 in total

1.  Involvement of murine β-1,4-galactosyltransferase V in lactosylceramide biosynthesis.

Authors:  Tadahiro Kumagai; Takeshi Sato; Shunji Natsuka; Yukito Kobayashi; Dapeng Zhou; Tadashi Shinkai; Satoru Hayakawa; Kiyoshi Furukawa
Journal:  Glycoconj J       Date:  2010-11-06       Impact factor: 2.916

2.  Structural and quantitative evidence for dynamic glycome shift on production of induced pluripotent stem cells.

Authors:  Kayo Hasehira; Hiroaki Tateno; Yasuko Onuma; Yuzuru Ito; Makoto Asashima; Jun Hirabayashi
Journal:  Mol Cell Proteomics       Date:  2012-09-27       Impact factor: 5.911

3.  Chain elongation of pectic beta-(1-->4)-galactan by a partially purified galactosyltransferase from soybean (Glycine max Merr.) hypocotyls.

Authors:  Tomoyuki Konishi; Toshihisa Kotake; Yoichi Tsumuraya
Journal:  Planta       Date:  2007-03-17       Impact factor: 4.540

4.  Structural and quantitative evidence of α2-6-sialylated N-glycans as markers of the differentiation potential of human mesenchymal stem cells.

Authors:  Kayo Hasehira; Jun Hirabayashi; Hiroaki Tateno
Journal:  Glycoconj J       Date:  2016-06-17       Impact factor: 2.916
  4 in total

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