BACKGROUND AND AIMS: Changes in some rhythmometric parameters have been reported in the elderly as a consequence of both structural and neurochemical changes occurring in the central nervous system. Since alterations of lipid and lipoprotein metabolism are directly involved in several age-related disorders, the aim of this study was to investigate the circadian temporal organization of some important lipidic fractions (total cholesterol, triacylglycerol, apolipoprotein A1 and B) in physiological aging. METHODS: Thirty old hospitalized subjects were synchronized for daily activities, sleeping/waking habits, and time/quality of meals. Twenty-four healthy young individuals served as controls. After an overnight fast, samples were taken beginning at 08:00 every 4 hours until 20:00, and every 2 hours from 20:00 to 04:00. Rhythmometric data were analyzed by single and population mean Cosinor analysis, and by ANOVA; the comparison of the rhythm's parameters between elderly and young subjects was carried out by the Mesor test and the amplitude-acrophase using Hotelling's test. RESULTS: Elderly subjects exhibited statistically significant circadian rhythms for total cholesterol (p<0.00002), triacylglycerol (p<0.000001), apo A-1 (p<0.0013), and apo B (p<O.0104). Young subjects also exhibited statistically significant daily fluctuations for total cholesterol (p<0. 0003), triacylglycerol (p<0. 03), apo A-1 (p<0.002) and apo B (p<0.003). The mean level of apo B rhythm was higher in old subjects than in controls. CONCLUSIONS: These data suggest that the circadian temporal organization of lipidic fractions is maintained in physiological aging and underline the importance of the feeding schedule as a powerful synchronizer of the daily lipidic profile.
BACKGROUND AND AIMS: Changes in some rhythmometric parameters have been reported in the elderly as a consequence of both structural and neurochemical changes occurring in the central nervous system. Since alterations of lipid and lipoprotein metabolism are directly involved in several age-related disorders, the aim of this study was to investigate the circadian temporal organization of some important lipidic fractions (total cholesterol, triacylglycerol, apolipoprotein A1 and B) in physiological aging. METHODS: Thirty old hospitalized subjects were synchronized for daily activities, sleeping/waking habits, and time/quality of meals. Twenty-four healthy young individuals served as controls. After an overnight fast, samples were taken beginning at 08:00 every 4 hours until 20:00, and every 2 hours from 20:00 to 04:00. Rhythmometric data were analyzed by single and population mean Cosinor analysis, and by ANOVA; the comparison of the rhythm's parameters between elderly and young subjects was carried out by the Mesor test and the amplitude-acrophase using Hotelling's test. RESULTS: Elderly subjects exhibited statistically significant circadian rhythms for total cholesterol (p<0.00002), triacylglycerol (p<0.000001), apo A-1 (p<0.0013), and apo B (p<O.0104). Young subjects also exhibited statistically significant daily fluctuations for total cholesterol (p<0. 0003), triacylglycerol (p<0. 03), apo A-1 (p<0.002) and apo B (p<0.003). The mean level of apo B rhythm was higher in old subjects than in controls. CONCLUSIONS: These data suggest that the circadian temporal organization of lipidic fractions is maintained in physiological aging and underline the importance of the feeding schedule as a powerful synchronizer of the daily lipidic profile.