Effect of ions on the activity of the enzyme alkaline phosphatase from Plectonema boryanum.

Alkaline phosphatase extracted from P. boryanum with lysozyme or polymyxin B treatment was used in a comparative study of cell bound and cell free enzyme. The effects of various ions on enzyme activity were tested. Calcium was found to enhance activity to the greatest degree stimulating the cell bound alkaline phosphatase 100% and cell free enzyme four-fold. Magnesium and potassium also stimulated the activity of cell bound and cell free enzyme. Other ions were found to be inhibitory to varying degrees.