Literature DB >> 12089475

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy.

T Venugopal1, S Mathavan, T J Pandian.   

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

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Year:  2002        PMID： 12089475     DOI： 10.1007/bf02704915

Source DB:  PubMed          Journal:  J Biosci        ISSN： 0250-5991            Impact factor:   1.826


  39 in total

1.  Improved performance in protein secondary structure prediction by inhomogeneous score combination.

Authors:  Y Guermeur; C Geourjon; P Gallinari; G Deléage
Journal:  Bioinformatics       Date:  1999-05       Impact factor: 6.937

2.  A heuristic approach to predicting the tertiary structure of bovine somatotropin.

Authors:  L Carlacci; K C Chou; G M Maggiora
Journal:  Biochemistry       Date:  1991-05-07       Impact factor: 3.162

3.  Purification and electrophoretic analysis of glycosylated chicken growth hormone (G-cGH): evidence of G-cGH isoforms.

Authors:  C Arámburo; S Navarrette; J L Montiel; R Sánchez; L R Berghman
Journal:  Gen Comp Endocrinol       Date:  1991-10       Impact factor: 2.822

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  A novel ligation mediated-PCR based strategy for construction of subtraction libraries from limiting amounts of mRNA.

Authors:  S Ghosh
Journal:  Nucleic Acids Res       Date:  1996-02-15       Impact factor: 16.971

6.  cDNA subtraction library construction using a magnet-assisted subtraction technique (MAST).

Authors:  P Schraml; R Shipman; P Stulz; C U Ludwig
Journal:  Trends Genet       Date:  1993-03       Impact factor: 11.639

7.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

8.  High-efficiency cloning of full-length cDNA.

Authors:  H Okayama; P Berg
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272

9.  Growth hormone-dependent potentiation of gonadotropin-stimulated steroid production by ovarian follicles of the goldfish.

Authors:  G Van der Kraak; P M Rosenblum; R E Peter
Journal:  Gen Comp Endocrinol       Date:  1990-08       Impact factor: 2.822

10.  Molecular phylogeny of bony fishes, based on the amino acid sequence of the growth hormone.

Authors:  G Bernardi; G D'Onofrio; S Caccio; G Bernardi
Journal:  J Mol Evol       Date:  1993-12       Impact factor: 2.395
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  1 in total

1.  The growth hormone-encoding gene isolated and characterized from Labeo rohita Hamilton is expressed in CHO cells under the control of constitutive promoters in 'autotransgene' constructs.

Authors:  R Rajesh; K C Majumdar
Journal:  Fish Physiol Biochem       Date:  2008-02-07       Impact factor: 2.794
  1 in total

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