Literature DB >> 12088882

Purification of antibodies by affinity chromatography.

Klaus Huse1, Hans-Joachim Böhme, Gerhard H Scholz.   

Abstract

This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody. The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described.

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Year:  2002        PMID: 12088882     DOI: 10.1016/s0165-022x(02)00017-9

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  17 in total

1.  Bead injection for biomolecular assays: Affinity chromatography enhanced by bead injection spectroscopy.

Authors:  Yan Gutzman; Andrea D Carroll; Jaromir Ruzicka
Journal:  Analyst       Date:  2006-06-06       Impact factor: 4.616

2.  Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G.

Authors:  Shin Miyakawa; Yusuke Nomura; Taiichi Sakamoto; Yoshiki Yamaguchi; Koichi Kato; Satoko Yamazaki; Yoshikazu Nakamura
Journal:  RNA       Date:  2008-04-25       Impact factor: 4.942

3.  Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits.

Authors:  Sadeq Eivazi; Jafar Majidi; Leili Aghebati Maleki; Jalal Abdolalizadeh; Mehdi Yousefi; Majid Ahmadi; Somayeh Dadashi; Zahra Moradi; Elmira Zolali
Journal:  Adv Pharm Bull       Date:  2015-03-05

4.  Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing.

Authors:  Nicholas E Levy; Kristin N Valente; Leila H Choe; Kelvin H Lee; Abraham M Lenhoff
Journal:  Biotechnol Bioeng       Date:  2013-12-11       Impact factor: 4.530

5.  Antibody Purification Using Affinity Chromatography.

Authors:  Arabelle Cassedy; Richard O'Kennedy
Journal:  Methods Mol Biol       Date:  2022

Review 6.  Expression of mammalian proteins for diagnostics and therapeutics: a review.

Authors:  Jacqueline Kar Kei Mark; Crystale Siew Ying Lim; Fazlina Nordin; Gee Jun Tye
Journal:  Mol Biol Rep       Date:  2022-06-08       Impact factor: 2.742

Review 7.  Immunofluorescence imaging of DNA damage response proteins: optimizing protocols for super-resolution microscopy.

Authors:  Brian T Bennett; Jörg Bewersdorf; Kendall L Knight
Journal:  Methods       Date:  2009-02-24       Impact factor: 3.608

8.  Ribosome display selection of a murine IgG₁ Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.

Authors:  Sebastian Grimm; Feifan Yu; Per-Åke Nygren
Journal:  Mol Biotechnol       Date:  2011-07       Impact factor: 2.695

9.  Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies.

Authors:  Maria Soler; M-Carmen Estevez; Mar Alvarez; Marinus A Otte; Borja Sepulveda; Laura M Lechuga
Journal:  Sensors (Basel)       Date:  2014-01-29       Impact factor: 3.576

10.  Novel antibody against a glutamic acid-rich human fibrinogen-like protein 2-derived peptide near Ser91 inhibits hfgl2 prothrombinase activity.

Authors:  Wen-Zhu Li; Jue Wang; Rui Long; Guan-Hua Su; Dinesh-Kumar Bukhory; Jing Dai; Nan Jin; Shi-Yuan Huang; Peng Jia; Ting Li; Chen Fan; Kun Liu; Zhaohui Wang
Journal:  PLoS One       Date:  2014-04-11       Impact factor: 3.240

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