OBJECTIVE: To present and contrast the results of immunohistochemistry and fluorescence in situ hybridization (FISH) proficiency testing surveys for HER-2/neu, as conducted by the Cell Markers and Cytogenetics Committees of the College of American Pathologists. DESIGN: During the past 2 years, unstained sections from invasive breast carcinomas have been used for both immunohistochemistry and interphase FISH proficiency surveys. In most instances, the same cases were used for both the Cell Markers and Cytogenetics surveys. Additional data regarding interpretative variability for immunohistochemistry surveys have also been captured. RESULTS: The number of laboratories performing FISH for HER-2/neu testing doubled during the 2-year period. The results of FISH testing have been remarkably concordant for laboratories submitting results. In contrast, the results of immunohistochemistry testing continue to highlight substantial variability among laboratories evaluating the same cases. The data show that this variability is at least in part due to variability in interpretation among observers, as well as inherent differences between immunohistochemistry and FISH techniques. CONCLUSIONS: College of American Pathologists proficiency survey programs provide useful information about clinical testing for HER-2/neu amplification/overexpression.
OBJECTIVE: To present and contrast the results of immunohistochemistry and fluorescence in situ hybridization (FISH) proficiency testing surveys for HER-2/neu, as conducted by the Cell Markers and Cytogenetics Committees of the College of American Pathologists. DESIGN: During the past 2 years, unstained sections from invasive breast carcinomas have been used for both immunohistochemistry and interphase FISH proficiency surveys. In most instances, the same cases were used for both the Cell Markers and Cytogenetics surveys. Additional data regarding interpretative variability for immunohistochemistry surveys have also been captured. RESULTS: The number of laboratories performing FISH for HER-2/neu testing doubled during the 2-year period. The results of FISH testing have been remarkably concordant for laboratories submitting results. In contrast, the results of immunohistochemistry testing continue to highlight substantial variability among laboratories evaluating the same cases. The data show that this variability is at least in part due to variability in interpretation among observers, as well as inherent differences between immunohistochemistry and FISH techniques. CONCLUSIONS: College of American Pathologists proficiency survey programs provide useful information about clinical testing for HER-2/neu amplification/overexpression.
Authors: Edith A Perez; Amylou C Dueck; Ann E McCullough; Monica M Reinholz; Kathleen S Tenner; Nancy E Davidson; Julie Gralow; Lyndsay N Harris; Leila A Kutteh; David W Hillman; Robert B Jenkins; Beiyun Chen Journal: J Natl Cancer Inst Date: 2011-12-02 Impact factor: 13.506
Authors: Raymond Tubbs; James Pettay; David Hicks; Marek Skacel; Richard Powell; Tom Grogan; James Hainfeld Journal: J Mol Histol Date: 2004-08 Impact factor: 2.611
Authors: Rachel Stevens; Imad Almanaseer; Miguel Gonzalez; Derin Caglar; Ryan A Knudson; Rhett P Ketterling; Daniel S Schrock; Thomas A Seemayer; Julia A Bridge Journal: J Mol Diagn Date: 2007-04 Impact factor: 5.568