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Literature DB >> 12088033

Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.

Won-Gun Koh¹, Alexander Revzin, Michael V Pishko.

Abstract

We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 micrometers in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 micrometers allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

Entities: Chemical Species

Keywords: NASA Discipline Life Sciences Technologies; Non-NASA Center

Mesh：

Substances：

Year: 2002 PMID： 12088033 DOI： 10.1021/la0115740

Source DB: PubMed Journal: Langmuir ISSN： 0743-7463 Impact factor: 3.882

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Cited

39 in total

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Authors: Matthew E Helgeson; Stephen C Chapin; Patrick S Doyle
Journal: Curr Opin Colloid Interface Sci Date: 2011-04-01 Impact factor: 6.448

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3. In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

Authors: Aleksander Skardal; Mahesh Devarasetty; Shay Soker; Adam R Hall
Journal: Biofabrication Date: 2015-09-10 Impact factor: 9.954

4. Laser-guided assembly of heterotypic three-dimensional living cell microarrays.

Authors: G M Akselrod; W Timp; U Mirsaidov; Q Zhao; C Li; R Timp; K Timp; P Matsudaira; G Timp
Journal: Biophys J Date: 2006-08-04 Impact factor: 4.033

5. Three-dimensional laser micro- and nano-structuring of acrylated poly(ethylene glycol) materials and evaluation of their cytoxicity for tissue engineering applications.

Authors: A Ovsianikov; M Malinauskas; S Schlie; B Chichkov; S Gittard; R Narayan; M Löbler; K Sternberg; K-P Schmitz; A Haverich
Journal: Acta Biomater Date: 2010-10-25 Impact factor: 8.947

Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.

1. Hydrogel microparticles from lithographic processes: novel materials for fundamental and applied colloid science.

Review 2. Designer nanoparticles: incorporating size, shape and triggered release into nanoscale drug carriers.

3. In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

4. Laser-guided assembly of heterotypic three-dimensional living cell microarrays.

5. Three-dimensional laser micro- and nano-structuring of acrylated poly(ethylene glycol) materials and evaluation of their cytoxicity for tissue engineering applications.

Review 6. Microscale technologies for tissue engineering and biology.

7. Biphasic electrical field stimulation aids in tissue engineering of multicell-type cardiac organoids.

Review 8. Cell encapsulation in biodegradable hydrogels for tissue engineering applications.

9. An adaptable hydrogel array format for 3-dimensional cell culture and analysis.

10. Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device.