Y Peng1, F Liu, J Luo, B Liu. 1. Department of Internal Medicine, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Abstract
OBJECTIVE: To investigate the effects of danshen(Salvia plectranthoides Griff.) and shengmaiye (Panax ginseng C. A. Mey, Ophiopogon japonicus Ker-Gawl and Schisandra chinensis Baill) on glomerulosclerosis induced by adriamycin in SD rats. METHODS: Left kidney of the animals was removed and after 7 days, adriamycin(6 mg.kg-1) was injected through tail vein, so as to establish an animal disease model. Then danshen or shengmaiye was injected to peritoneal cavity of the rats. All rats were killed by the end of the 8th week. Hemoglobin, BUN, cholesterol and protein in urea within 24 hours(ur Pro/24 h) were detected. IV collagen(IV col.) and laminin(LN) of renal cortex were determined by ELISA. The quantitation of IV col and LN in glomerular mesangial area was analyzed by computer pictures. RESULTS: Compared with model-danshen group(Group III), model-shenmaiye group(Group IV) and normal control group(Group I), BUN, cholesterol(Ch) and ur Pro/24 h were increased obviously and hemoglobin(Hb) was decreased in model control group(Group II). The values of BUN, Ch and Ur Pro were lower in Group III and Group IV than those in Group II and higher than those in Group I (P < 0.05). The quantitation of IV col and LN within renal cortex and mesangial area was less in Group III and Group IV than that in Group II and more than that in Group I (P < 0.05 or P < 0.01). CONCLUSION: Dansheng and shengmaiye may play an important role in the treatment of glomerulosclerosis in rats.
OBJECTIVE: To investigate the effects of danshen(Salvia plectranthoides Griff.) and shengmaiye (Panax ginseng C. A. Mey, Ophiopogon japonicus Ker-Gawl and Schisandra chinensis Baill) on glomerulosclerosis induced by adriamycin in SD rats. METHODS: Left kidney of the animals was removed and after 7 days, adriamycin(6 mg.kg-1) was injected through tail vein, so as to establish an animal disease model. Then danshen or shengmaiye was injected to peritoneal cavity of the rats. All rats were killed by the end of the 8th week. Hemoglobin, BUN, cholesterol and protein in urea within 24 hours(ur Pro/24 h) were detected. IV collagen(IV col.) and laminin(LN) of renal cortex were determined by ELISA. The quantitation of IV col and LN in glomerular mesangial area was analyzed by computer pictures. RESULTS: Compared with model-danshen group(Group III), model-shenmaiye group(Group IV) and normal control group(Group I), BUN, cholesterol(Ch) and ur Pro/24 h were increased obviously and hemoglobin(Hb) was decreased in model control group(Group II). The values of BUN, Ch and Ur Pro were lower in Group III and Group IV than those in Group II and higher than those in Group I (P < 0.05). The quantitation of IV col and LN within renal cortex and mesangial area was less in Group III and Group IV than that in Group II and more than that in Group I (P < 0.05 or P < 0.01). CONCLUSION: Dansheng and shengmaiye may play an important role in the treatment of glomerulosclerosis in rats.
Authors: Zeping Hu; Xiaoxia Yang; Paul Chi Lui Ho; Sui Yung Chan; Paul Wan Sia Heng; Eli Chan; Wei Duan; Hwee Ling Koh; Shufeng Zhou Journal: Drugs Date: 2005 Impact factor: 9.546