Literature DB >> 12079331

A novel bacteriophage-encoded RNA polymerase binding protein inhibits transcription initiation and abolishes transcription termination by host RNA polymerase.

Sergei Nechaev1, Yulia Yuzenkova, Anita Niedziela-Majka, Tomasz Heyduk, Konstantin Severinov.   

Abstract

Xp10 is a lytic bacteriophage of Xanthomonas oryzae, a Gram-negative bacterium that causes rice blight. We purified an Xp10 protein, p7, that binds to and inhibits X. oryzae RNA polymerase (RNAP). P7 is a novel 73 amino acid-long protein; it does not bind to and hence does not affect transcription by Escherichia coli RNAP. Analysis of E. coli/X. oryzae RNAP hybrids locates the p7 binding site to the largest X. oryzae RNAP subunit, beta'. Binding of p7 to X. oryzae RNAP holoenzyme prevents large conformational change that places the sigma subunit region 4 into the correct position for interaction with the -35 promoter element. As a result, open promoter complex formation on the -10/-35 class promoters is inhibited. Inhibition of promoter complex formation on the extended -10 class promoters is less efficient. The p7 protein also abolishes factor-independent transcription termination by X. oryzae RNAP by preventing the release of nascent RNA at terminators. Further physiological and mechanistic studies of this novel transcription factor should provide additional insights into its biological role and the processes of promoter recognition and transcription termination. (c) 2002 Elsevier Science Ltd.

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Year:  2002        PMID: 12079331     DOI: 10.1016/S0022-2836(02)00420-5

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  10 in total

1.  Distinct pathways of RNA polymerase regulation by a phage-encoded factor.

Authors:  Daria Esyunina; Evgeny Klimuk; Konstantin Severinov; Andrey Kulbachinskiy
Journal:  Proc Natl Acad Sci U S A       Date:  2015-02-02       Impact factor: 11.205

2.  T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.

Authors:  Beatriz Cámara; Minhao Liu; Jonathan Reynolds; Andrey Shadrin; Bing Liu; King Kwok; Peter Simpson; Robert Weinzierl; Konstantin Severinov; Ernesto Cota; Steve Matthews; Siva R Wigneshweraraj
Journal:  Proc Natl Acad Sci U S A       Date:  2010-01-19       Impact factor: 11.205

3.  Mapping of RNA polymerase residues that interact with bacteriophage Xp10 transcription antitermination factor p7.

Authors:  Yulia Yuzenkova; Nikolay Zenkin; Konstantin Severinov
Journal:  J Mol Biol       Date:  2007-10-25       Impact factor: 5.469

4.  Molecular evolution of multisubunit RNA polymerases: structural analysis.

Authors:  William J Lane; Seth A Darst
Journal:  J Mol Biol       Date:  2009-11-03       Impact factor: 5.469

5.  The sabotage of the bacterial transcription machinery by a small bacteriophage protein.

Authors:  Bing Liu; Andrey Shadrin; Carol Sheppard; Vladimir Mekler; Yingqi Xu; Konstantin Severinov; Steve Matthews; Sivaramesh Wigneshweraraj
Journal:  Bacteriophage       Date:  2014-03-12

6.  A novel phage-encoded transcription antiterminator acts by suppressing bacterial RNA polymerase pausing.

Authors:  Zhanna Berdygulova; Daria Esyunina; Nataliya Miropolskaya; Damir Mukhamedyarov; Konstantin Kuznedelov; Bryce E Nickels; Konstantin Severinov; Andrey Kulbachinskiy; Leonid Minakhin
Journal:  Nucleic Acids Res       Date:  2012-01-11       Impact factor: 16.971

7.  Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase.

Authors:  Nikolay Zenkin; Konstantin Severinov; Yulia Yuzenkova
Journal:  Nucleic Acids Res       Date:  2015-06-01       Impact factor: 16.971

8.  A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement.

Authors:  Bing Liu; Andrey Shadrin; Carol Sheppard; Vladimir Mekler; Yingqi Xu; Konstantin Severinov; Steve Matthews; Sivaramesh Wigneshweraraj
Journal:  Nucleic Acids Res       Date:  2014-01-30       Impact factor: 16.971

9.  Transcription Profiling of Bacillus subtilis Cells Infected with AR9, a Giant Phage Encoding Two Multisubunit RNA Polymerases.

Authors:  Daria Lavysh; Maria Sokolova; Marina Slashcheva; Konrad U Förstner; Konstantin Severinov
Journal:  MBio       Date:  2017-02-14       Impact factor: 7.867

10.  Structural basis for transcription antitermination at bacterial intrinsic terminator.

Authors:  Linlin You; Jing Shi; Liqiang Shen; Lingting Li; Chengli Fang; Chengzhi Yu; Wenbo Cheng; Yu Feng; Yu Zhang
Journal:  Nat Commun       Date:  2019-07-11       Impact factor: 14.919

  10 in total

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