A comparative analysis of invaded sequences from group IA phospholipase A(2) genes provides evidence about the divergence period of genes groups and snake families.

Two phospholipase A(2) (PLA(2)) genes classified into group IA were cloned from the genomic library of the sea snake Laticauda semifasciata. Eight clones were obtained by PCR cloning procedure from genomic DNA of Laticauda laticaudata (four clones) and Laticauda colubrina (four clones). The genes were 3.6-4.4kbp in length. Intron and exon organization of the group IA PLA(2) genes was the same as that of Naja sputatrix group IA PLA(2) genes (four exons and three introns). There were two kinds of repetitive sequences in the first and second introns of all sequenced PLA(2) genes. The differences in the length of these genes were derived from the length of their repetitive sequences. The chicken repeat-1 (CR1)-like long interspersed repeated DNA (LINE) sequences, different from the above repetitive sequences, were also found in all sequenced Laticauda PLA(2) genes. A comparative analysis of groups IA, IA' and IIA PLA(2)s genes suggests a period of CR1-like LINE integration during molecular and family evolution. The integration of CR1-like LINE into PLA(2) genes occurred after the divergence of groups I and II PLA(2)s but before the divergence of groups, IA and IA' PLA(2)s. These integration events occurred before the family divergence of Naja and Laticauda. The presence of CR1-like LINE and a comparison of intron and exon organization showed that the divergence of Naja and Bungarus occurred before the divergence of Laticauda and Naja.