Is novel wheel inhibition of per1 and per2 expression linked to phase shift occurrence?

We studied whether access to a novel running wheel in vivo could reset the suprachiasmatic nuclei (SCN) in vitro. Golden hamsters were transferred to dim red light at Zeitgeber time (ZT) 4, given their first exposure to a running wheel for 3 h, and killed at either ZT7 or ZT9. Using a brain slice preparation, the SCN firing rate rhythm in vitro was advanced relative to controls only in the slices prepared at ZT9 (phase shift: 2.36+/-0.06 h, n=4) but not ZT7 (-0.26+/-0.16 h, n=4). Transitions to dim red light or brain slice preparation at ZT7 or ZT9 alone do not shift the rhythm. Hamsters with wheels had significantly lower levels of SCN per1 mRNA compared with controls at ZT7, and lower per2 mRNA when examined at ZT9. We conclude that 3 h of novel wheel access appears to require some extended time in vivo in order for the SCN to be reset, even beyond the time when per1 mRNA levels have been altered.