| Literature DB >> 12073087 |
Yukiko Kodama1, Fumihiko Omura, Keiko Takahashi, Katsuhiko Shirahige, Toshihiko Ashikari.
Abstract
Saccharomyces cerevisiae Ssy1p is a membrane protein which senses extracellular amino acids and controls the expression of certain amino acid permease genes. Analysis by DNA micro-array newly identified DIP5 and MUP1 as the positive targets and CAN1, PUT4 and GAP1 as the negative targets under Ssy1p control. Interestingly, the effect of ssy1 deletion was not restricted to amino acid permease genes: the expression of nitrogen catabolite repression (NCR)-sensitive genes and methionine-biosynthesizing genes ( MET genes) was derepressed by the deletion of SSY1. Constitutive overexpression of the genes for glutamine permease ( GNP1) or methionine permease ( MUP1) enhanced the assimilation of glutamine or methionine in the ssy1Delta strain but could not fully suppress the derepression of the NCR-sensitive genes or MET genes. This result suggests that Ssy1p regulates not only the transcription of amino acid permease genes, but also transcription of many other nitrogen-metabolizing genes.Entities:
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Year: 2002 PMID： 12073087 DOI： 10.1007/s00294-002-0291-1
Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886