| Literature DB >> 12072504 |
Abstract
The possible biochemical factors able to affect the in vitro expression of the high-risk human papillomavirus type 16 (HPV16) E7 oncoprotein have been analyzed. Evidence is provided that E7 mRNA stability is increased and, conversely, transcript translation is inhibited by binding to a 32-kDa protein from rabbit reticulocyte lysate; sequence analysis identified the 32-kDa binding protein as rabbit alpha(1)-globin protein; and interaction between rabbit alpha(1)-globin and E7 mRNA occurs through the 6-mer peptide SEQIKA present in human cytokeratin 7 protein. The in vitro data were confirmed by the occurrence of HPV16 E7 mRNA-cytokeratin 7 binding in squamous cervical cancer SiHa cells.Entities:
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Year: 2002 PMID: 12072504 PMCID: PMC136328 DOI: 10.1128/jvi.76.14.7040-7048.2002
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103