Literature DB >> 12069419

Separation of synthetic oligonucleotide dithioates from monothiophosphate impurities by anion-exchange chromatography on a mono-q column.

Xianbin Yang1, Richard P Hodge, Bruce A Luxon, Robert Shope, David G Gorenstein.   

Abstract

A method using a strong anion-exchange liquid-chromatography column, Mono-Q, has been developed for high-resolution analysis and purification of oligonucleotide dithioates, which were synthesized by an automated, solid-phase, phosphorothioamidite chemistry. High-resolution separation of oligonucleotide phosphorodithioates from monothiophosphate impurities was obtained. High-resolution separation was also demonstrated at pH 8. The separation of oligonucleotide dithioates was found to be linearly dependent on the number of sulfurs for the same sequence length. Thiocyanate, SCN-, as eluting anion, can be used to purify oligonucleotides containing a high percentage of phosphorodithioate linkages in lower salt concentrations and provides better separation than chloride as eluting anion.

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Year:  2002        PMID： 12069419     DOI： 10.1006/abio.2001.5694

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Construction and selection of bead-bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries designed for rapid PCR-based sequencing.

Authors:  Xianbin Yang; Suzanne E Bassett; Xin Li; Bruce A Luxon; Norbert K Herzog; Robert E Shope; Judy Aronson; Tarl W Prow; James F Leary; Romy Kirby; Andrew D Ellington; David G Gorenstein
Journal:  Nucleic Acids Res       Date:  2002-12-01       Impact factor: 16.971

2.  2'-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activity.

Authors:  Sherry Y Wu; Xianbin Yang; Kshipra M Gharpure; Hiroto Hatakeyama; Martin Egli; Michael H McGuire; Archana S Nagaraja; Takahito M Miyake; Rajesha Rupaimoole; Chad V Pecot; Morgan Taylor; Sunila Pradeep; Malgorzata Sierant; Cristian Rodriguez-Aguayo; Hyun J Choi; Rebecca A Previs; Guillermo N Armaiz-Pena; Li Huang; Carlos Martinez; Tom Hassell; Cristina Ivan; Vasudha Sehgal; Richa Singhania; Hee-Dong Han; Chang Su; Ji Hoon Kim; Heather J Dalton; Chandra Kovvali; Khandan Keyomarsi; Nigel A J McMillan; Willem W Overwijk; Jinsong Liu; Ju-Seog Lee; Keith A Baggerly; Gabriel Lopez-Berestein; Prahlad T Ram; Barbara Nawrot; Anil K Sood
Journal:  Nat Commun       Date:  2014-03-12       Impact factor: 14.919

3.  Solid-Phase Synthesis of RNA Analogs Containing Phosphorodithioate Linkages.

Authors:  Xianbin Yang
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2017-09-18

4.  Crystal structures of thrombin in complex with chemically modified thrombin DNA aptamers reveal the origins of enhanced affinity.

Authors:  Rafal Dolot; Curtis H Lam; Malgorzata Sierant; Qiang Zhao; Feng-Wu Liu; Barbara Nawrot; Martin Egli; Xianbin Yang
Journal:  Nucleic Acids Res       Date:  2018-05-18       Impact factor: 16.971

5.  Gene silencing activity of siRNA molecules containing phosphorodithioate substitutions.

Authors:  Xianbin Yang; Malgorzata Sierant; Magdalena Janicka; Lukasz Peczek; Carlos Martinez; Tom Hassell; Na Li; Xin Li; Tianzhi Wang; Barbara Nawrot
Journal:  ACS Chem Biol       Date:  2012-04-26       Impact factor: 5.100

6.  Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers.

Authors:  Xianbin Yang; Xin Li; Tarl W Prow; Lisa M Reece; Suzanne E Bassett; Bruce A Luxon; Norbert K Herzog; Judy Aronson; Robert E Shope; James F Leary; David G Gorenstein
Journal:  Nucleic Acids Res       Date:  2003-05-15       Impact factor: 16.971

7.  Entropic Enhancement of Protein-DNA Affinity by Oxygen-to-Sulfur Substitution in DNA Phosphate.

Authors:  Levani Zandarashvili; Dan Nguyen; Kurtis M Anderson; Mark A White; David G Gorenstein; Junji Iwahara
Journal:  Biophys J       Date:  2015-09-01       Impact factor: 4.033

Review 8.  Development of Phosphorothioate DNA and DNA Thioaptamers.

Authors:  David E Volk; Ganesh L R Lokesh
Journal:  Biomedicines       Date:  2017-07-13

Review 9.  Oligonucleotides Isolation and Separation-A Review on Adsorbent Selection.

Authors:  Sylwia Studzińska; Łukasz Nuckowski; Bogusław Buszewski
Journal:  Int J Mol Sci       Date:  2022-08-23       Impact factor: 6.208

10.  Evoking picomolar binding in RNA by a single phosphorodithioate linkage.

Authors:  N Dinuka Abeydeera; Martin Egli; Nehemiah Cox; Karen Mercier; Jonas Nascimento Conde; Pradeep S Pallan; Daniella M Mizurini; Malgorzata Sierant; Fatima-Ezzahra Hibti; Tom Hassell; Tianzhi Wang; Feng-Wu Liu; Hong-Min Liu; Carlos Martinez; Anil K Sood; Terry P Lybrand; Chiraz Frydman; Robson Q Monteiro; Richard H Gomer; Barbara Nawrot; Xianbin Yang
Journal:  Nucleic Acids Res       Date:  2016-08-26       Impact factor: 16.971
  10 in total

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