Literature DB >> 12063390

Microwave decondensation and codenaturation: a new methodology to maximize FISH data from donors with very low concentrations of sperm.

E Ko1, A Rademaker, R Martin.   

Abstract

To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques. Copyright 2002 S. Karger AG, Basel

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Mesh：

Year:  2001        PMID： 12063390     DOI： 10.1159/000059336

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  7 in total

1.  Unusual segregation products in sperm from a pericentric inversion 17 heterozygote.

Authors:  Monica M Mikhaail-Philips; Barbara C McGillivray; Sara J Hamilton; Evelyn Ko; Judy Chernos; Alfred Rademaker; Renée H Martin
Journal:  Hum Genet       Date:  2005-05-28       Impact factor: 4.132

2.  Fluorescent in situ hybridization for sex chromosome determination before and after fertilization in mice.

Authors:  J J Whyte; R M Roberts; C S Rosenfeld
Journal:  Theriogenology       Date:  2007-01-09       Impact factor: 2.740

3.  Human male recombination maps for individual chromosomes.

Authors:  Fei Sun; Maria Oliver-Bonet; Thomas Liehr; Heike Starke; Evelyn Ko; Alfred Rademaker; Joaquima Navarro; Jordi Benet; Renée H Martin
Journal:  Am J Hum Genet       Date:  2004-02-18       Impact factor: 11.025

Review 4.  Is there a relationship between sperm chromosome abnormalities and sperm morphology?

Authors:  Fei Sun; Evelyn Ko; Renée H Martin
Journal:  Reprod Biol Endocrinol       Date:  2006-01-25       Impact factor: 5.211

5.  Reduced meiotic recombination on the XY bivalent is correlated with an increased incidence of sex chromosome aneuploidy in men with non-obstructive azoospermia.

Authors:  F Sun; M Mikhaail-Philips; M Oliver-Bonet; E Ko; A Rademaker; P Turek; R H Martin
Journal:  Mol Hum Reprod       Date:  2008-06-26       Impact factor: 4.025

6.  Distribution of the sex chromosome during mouse spermatogenesis in testis tissue sections.

Authors:  Kosuke Otaka; Yuuki Hiradate; Norio Kobayashi; Yoshiki Shirakata; Kentaro Tanemura
Journal:  J Reprod Dev       Date:  2015-06-14       Impact factor: 2.214

7.  Sexing of dog sperm by fluorescence in situ hybridization.

Authors:  Maya Oi; Keisuke Yamada; Hiroyuki Hayakawa; Hiroshi Suzuki
Journal:  J Reprod Dev       Date:  2012-10-11       Impact factor: 2.214
  7 in total

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