Literature DB >> 12062428

Evidence that the putative alpha-glucosidase of Thermotoga maritima MSB8 is a pNP alpha-D-glucuronopyranoside hydrolyzing alpha-glucuronidase.

Cuddapah Suresh1, Ahmed Abu Rus'd, Motomitsu Kitaoka, Kiyoshi Hayashi.   

Abstract

The gene (agu) encoding p-nitrophenyl alpha-D-glucuronopyranoside (pNP-GUA) hydrolyzing alpha-glucuronidase of the hyperthermophilic bacterium Thermotoga maritima was cloned and expressed in Escherichia coli. The recombinant enzyme was purified and characterized. The gene previously designated as putative alpha-glucosidase was found to code for a protein that had no alpha-glucosidase activity. It showed a rare activity profile with its ability to hydrolyze pNP-GUA, an activity not known in the alpha-glucuronidases from microbial sources. This is the first report on the occurrence of an alpha-glucuronidase which belongs to the family 4 of glycosyl hydrolases.

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Year:  2002        PMID： 12062428     DOI： 10.1016/s0014-5793(02)02611-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  The Thermotoga maritima phenotype is impacted by syntrophic interaction with Methanococcus jannaschii in hyperthermophilic coculture.

Authors:  M R Johnson; S B Conners; C I Montero; C J Chou; K R Shockley; R M Kelly
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

2.  α-Galacturonidase(s): a new class of Family 4 glycoside hydrolases with strict specificity and a unique CHEV active site motif.

Authors:  John Thompson; Andreas Pikis; Jamie Rich; Barry G Hall; Stephen G Withers
Journal:  FEBS Lett       Date:  2013-02-14       Impact factor: 4.124

3.  An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima.

Authors:  Shannon B Conners; Clemente I Montero; Donald A Comfort; Keith R Shockley; Matthew R Johnson; Swapnil R Chhabra; Robert M Kelly
Journal:  J Bacteriol       Date:  2005-11       Impact factor: 3.490

4.  Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations.

Authors:  Barry G Hall; Andreas Pikis; John Thompson
Journal:  Mol Biol Evol       Date:  2009-07-22       Impact factor: 16.240

5.  Biochemical and Structural Characterization of a Five-domain GH115 α-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T.

Authors:  Weijun Wang; Ruoyu Yan; Boguslaw P Nocek; Thu V Vuong; Rosa Di Leo; Xiaohui Xu; Hong Cui; Paul Gatenholm; Guillermo Toriz; Maija Tenkanen; Alexei Savchenko; Emma R Master
Journal:  J Biol Chem       Date:  2016-04-18       Impact factor: 5.157

6.  Tagaturonate-fructuronate epimerase UxaE, a novel enzyme in the hexuronate catabolic network in Thermotoga maritima.

Authors:  Irina A Rodionova; David A Scott; Nick V Grishin; Andrei L Osterman; Dmitry A Rodionov
Journal:  Environ Microbiol       Date:  2012-08-23       Impact factor: 5.491

7.  Several archaeal homologs of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars.

Authors:  Dhaval M Nanavati; Kamolwan Thirangoon; Kenneth M Noll
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792
  7 in total

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